Astrocytes produce CNTF during the remyelination phase of viral-induced spinal cord demyelination to stimulate FGF-2 production

Phillip J Albrecht; Joshua C Murtie; Jennifer K Ness; Jeffrey M Redwine; Jonathan R Enterline; Regina C Armstrong; Steven W Levison

Neurobiology of Disease (Jul 2003)

Astrocytes produce CNTF during the remyelination phase of viral-induced spinal cord demyelination to stimulate FGF-2 production

Phillip J Albrecht,
Joshua C Murtie,
Jennifer K Ness,
Jeffrey M Redwine,
Jonathan R Enterline,
Regina C Armstrong,
Steven W Levison

Affiliations

Phillip J Albrecht: Department of Neuroscience & Anatomy, Pennsylvania State University College of Medicine, Hershey, PA 17033, USA; Department of Anatomy, Physiology, and Genetics, Uniformed Services University of the Health Sciences, Bethesda, MD, USA; Neurome Inc., 11149 N. Torrey Pines Rd., La Jolla, CA, USA
Joshua C Murtie: Department of Neuroscience & Anatomy, Pennsylvania State University College of Medicine, Hershey, PA 17033, USA; Department of Anatomy, Physiology, and Genetics, Uniformed Services University of the Health Sciences, Bethesda, MD, USA; Neurome Inc., 11149 N. Torrey Pines Rd., La Jolla, CA, USA
Jennifer K Ness: Department of Neuroscience & Anatomy, Pennsylvania State University College of Medicine, Hershey, PA 17033, USA; Department of Anatomy, Physiology, and Genetics, Uniformed Services University of the Health Sciences, Bethesda, MD, USA; Neurome Inc., 11149 N. Torrey Pines Rd., La Jolla, CA, USA
Jeffrey M Redwine: Department of Neuroscience & Anatomy, Pennsylvania State University College of Medicine, Hershey, PA 17033, USA; Department of Anatomy, Physiology, and Genetics, Uniformed Services University of the Health Sciences, Bethesda, MD, USA; Neurome Inc., 11149 N. Torrey Pines Rd., La Jolla, CA, USA
Jonathan R Enterline: Department of Neuroscience & Anatomy, Pennsylvania State University College of Medicine, Hershey, PA 17033, USA; Department of Anatomy, Physiology, and Genetics, Uniformed Services University of the Health Sciences, Bethesda, MD, USA; Neurome Inc., 11149 N. Torrey Pines Rd., La Jolla, CA, USA
Regina C Armstrong: Department of Neuroscience & Anatomy, Pennsylvania State University College of Medicine, Hershey, PA 17033, USA; Department of Anatomy, Physiology, and Genetics, Uniformed Services University of the Health Sciences, Bethesda, MD, USA; Neurome Inc., 11149 N. Torrey Pines Rd., La Jolla, CA, USA
Steven W Levison: Department of Neuroscience & Anatomy, Pennsylvania State University College of Medicine, Hershey, PA 17033, USA; Department of Anatomy, Physiology, and Genetics, Uniformed Services University of the Health Sciences, Bethesda, MD, USA; Neurome Inc., 11149 N. Torrey Pines Rd., La Jolla, CA, USA

Journal volume & issue: Vol. 13, no. 2
pp. 89 – 101

Abstract

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Multiple sclerosis is characterized by multiple lesions with selective loss of myelin and oligodendrocytes, leading to deficits of sensation and movement, as well as cognitive disabilities. Consequently, a major research endeavor is to identify strategies to enhance oligodendrocyte regeneration and remyelination. FGF-2 is a potent mitogen for OPCs, and it is induced in astrocytes in animal models of demyelinating diseases in conjunction with successful remyelination. However, the factors responsible for inducing FGF-2 after demyelination in astrocytes are unknown. Here we show that CNTF mRNA and protein increase coincident with spinal cord remyelination in mice recovering from MHV-induced demyelination. We identify CNTF within astrocytes surrounding and within remyelinating lesions, and show that CNTF increases FGF-2 ligand and receptor mRNAs in spinal cord after direct application. Furthermore, we show that CNTF increases FGF-2 mRNA approximately 2.5-fold in cultured mouse spinal cord astrocytes. Altogether, these results strongly implicate CNTF as an important cytokine in demyelinating disease and as an upstream regulator of FGF-2 production in astrocytes during early remyelination.

Published in Neurobiology of Disease

ISSN: 0969-9961 (Print); 1095-953X (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Medicine: Internal medicine: Neurosciences. Biological psychiatry. Neuropsychiatry
Website: https://www.journals.elsevier.com/neurobiology-of-disease

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