Hypertension in Pregnancy (Dec 2023)

Expression of NEDD4L and ENaC in Urinary Extracellular Vesicles in Pre-eclampsia

  • P.Y.M. Leung,
  • M Katerelos,
  • S. Choy,
  • N Cook,
  • M Lee,
  • K Paizis,
  • A Abboud,
  • J. A. Manning,
  • P.F Mount,
  • D.A Power

DOI
https://doi.org/10.1080/10641955.2023.2232029
Journal volume & issue
Vol. 42, no. 1

Abstract

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Objective To assess changes in expression of renal epithelial sodium channel (ENaC) and NEDD4L, a ubiquitin ligase, in urinary extracellular vesicles (UEV) of pre-eclamptic women compared to normal pregnant controls. Methods Urine was collected from pre-eclamptic women (PE, n = 20) or during normal pregnancy (NP, n = 20). UEV were separated by differential ultracentrifugation. NEDD4L, α-ENaC and γ-ENaC were identified by immunoblotting. Results There was no difference in the expression of NEDD4L (p = 0.17) and α-ENaC (p = 0.10). PE subjects showed increased expression of γ-ENaC by 6.9-fold compared to NP (p < 0.0001). Conclusion ENaC expression is upregulated in UEV of pre-eclamptic subjects but was not associated with changes in NEDD4L.

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