Leaf extracts of <i>Nandina domestica</i> Thunb ameliorate atopic dermatitis in NC/Nga mice treated with 1-chloro-2,4-dinitrobenzene (DNCB)

Abstract

Atopic dermatitis (AD) is a skin disease. In this study, we observed the effects of Nandina domestica (ND) on AD-induced NC/Nga mice with 1-chloro-2,4-dinitrobenzene (DNCB)-treatment. ND significantly ameliorated the AD-skin signs with reducing of skin thickness and mast cell infiltration in the injuried skin of DNCB-induced AD model. Furthermore, ND reduced serum immunoglobulin E (IgE) levels and restored into normal condition the total cell number (TCN) in dorsal skin tissue, the axillary lymph node (ALN), and spleen after DNCB exposure. ND also decreased the number of CD23+/B220+ cells in the ALN and CD3+ cells in dorsal skin. Moreover, it decreased the interleukin (IL)-4, (IL)-5 and IL-13 levels but increased the interferon-γ levels in splenocytes. Immunohistofluorescence staining showed that ND significantly increased claudin1 and Sirt1 protein expressions in the AD-induced mice. Our results suggested that ND significantly ameliorates DCNB-induced AD.

Keywords

• atopic dermatitis
• nandina domestica
• potential remedy
• immunoglobulin e
• dorsal skin tissue