Enzyme Immobilization by Inkjet Printing on Reagentless Biosensors for Electrochemical Phosphate Detection
Dongxing Zhang,
Yang Bai,
Haoran Niu,
Lingyun Chen,
Junfeng Xiao,
Qiuquan Guo,
Peipei Jia
Affiliations
Dongxing Zhang
Shenzhen Institute for Advanced Study, University of Electronic Science and Technology of China, Yesun Industry Zone, Guanlan Street, Shenzhen 518110, China
Yang Bai
Department of Biomedical Engineering, Western University, 1151 Richmond Street, London, ON N6A 3K7, Canada
Haoran Niu
Shenzhen Institute for Advanced Study, University of Electronic Science and Technology of China, Yesun Industry Zone, Guanlan Street, Shenzhen 518110, China
Lingyun Chen
Shenzhen Institute for Advanced Study, University of Electronic Science and Technology of China, Yesun Industry Zone, Guanlan Street, Shenzhen 518110, China
Junfeng Xiao
Shenzhen Institute for Advanced Study, University of Electronic Science and Technology of China, Yesun Industry Zone, Guanlan Street, Shenzhen 518110, China
Qiuquan Guo
Shenzhen Institute for Advanced Study, University of Electronic Science and Technology of China, Yesun Industry Zone, Guanlan Street, Shenzhen 518110, China
Peipei Jia
Shenzhen Institute for Advanced Study, University of Electronic Science and Technology of China, Yesun Industry Zone, Guanlan Street, Shenzhen 518110, China
Enzyme-based biosensors commonly utilize the drop-casting method for their surface modification. However, the drawbacks of this technique, such as low reproducibility, coffee ring effects, and challenges in mass production, hinder its application. To overcome these limitations, we propose a novel surface functionalization strategy of enzyme crosslinking via inkjet printing for reagentless enzyme-based biosensors. This method includes printing three functional layers onto a screen-printed electrode: the enzyme layer, crosslinking layer, and protective layer. Nanomaterials and substrates are preloaded together during our inkjet printing. Inkjet-printed electrodes feature a uniform enzyme deposition, ensuring high reproducibility and superior electrochemical performance compared to traditional drop-casted ones. The resultant biosensors display high sensitivity, as well as a broad linear response in the physiological range of the serum phosphate. This enzyme crosslinking method has the potential to extend into various enzyme-based biosensors through altering functional layer components.
