Protocol for human brain organoid transplantation into a rat visual cortex to model neural repair

Abstract

Summary: Human stem-cell-derived organoids represent a promising substrate for transplantation-based neural repair. Here, we describe a protocol for transplanting forebrain organoids into an injured adult rat visual cortex. This protocol includes surgical details for craniectomy, aspiration injury, organoid transplantation, and cranioplasty. This platform represents a valuable tool for investigating the efficacy of organoids as structured grafts for neural repair.For complete details on the use and execution of this protocol, please refer to Jgamadze et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Keywords

• Model Organisms
• Neuroscience
• Stem Cells
• Organoids