Stress Increases Ecological Risk of Glufosinate-Resistant Transgene Located on Alien Chromosomes in Hybrids Between Transgenic <i>Brassica napus</i> and Wild <i>Brassica juncea</i>

Abstract

When glufosinate-resistant transgenic Brassica napus (transgene PAT located on C chromosome) were backcrossed with wild Brassica juncea, 50% of the progeny expressed PAT under favourable conditions. However, exposure to stress (drought, salt, flooding, and intraspecific competition) increased the proportion of plants expressing the PAT gene (r-e plants) by approximately 20% compared to those under unstressed conditions. In the self-pollinated progeny of the stressed plants, the proportion of r-e plants increased by a nearly 30% compared to that of the unstressed plants. Composite fitness was comparable between plants developed under drought stress at the seedling stage and those grown under favourable conditions. Abscisic acid (ABA) content and expression of the Repressor of Silencing 1 (ROS1) in leaves increased significantly after stress treatment in the progeny, with r-e plants exhibiting higher levels. Exogenous ABA treatment significantly up-regulated ROS1 expression in progeny leaves, and the ABA treatment of seeds increased the survival of progeny exposed to glufosinate by 15%. Results suggest that increasing ABA under stress may enhance the demethylation of PAT’s promoter by promoting ROS1 expression, thereby inhibiting transgene silencing of PAT, indicating that transgene located on the C chromosome of transgenic B. napus may pose a higher risk of gene flow to wild B. juncea under stress, especially drought stress.

Keywords

• <i>Brassica juncea</i>
• Brassica napus
• gene silencing
• methylation
• stress
• herbicide resistant