Production of Polyhydroxyalkanoates by <i>Bacillus megaterium</i>: Prospecting on Rice Hull and Residual Glycerol Potential
Vanessa Kristine de Oliveira Schmidt,
Evelise Fonseca dos Santos,
Débora de Oliveira,
Marco Antônio Záchia Ayub,
Karina Cesca,
Paulo Roberto Dall Cortivo,
Cristiano José de Andrade,
Lilian Raquel Hickert
Affiliations
Vanessa Kristine de Oliveira Schmidt
Department of Chemical Engineering and Food Engineering, Federal University of Santa Catarina (EQA/UFSC), Campus Reitor João David Ferreira Lima-Centro Tecnológico, Florianópolis 88040-970, SC, Brazil
Evelise Fonseca dos Santos
Materials Recovery and Treatment Laboratory, State University of Rio Grande do Sul State (UERGS), R. Washington Luiz, 675-Centro Histórico, Porto Alegre 90010-460, RS, Brazil
Débora de Oliveira
Department of Chemical Engineering and Food Engineering, Federal University of Santa Catarina (EQA/UFSC), Campus Reitor João David Ferreira Lima-Centro Tecnológico, Florianópolis 88040-970, SC, Brazil
Marco Antônio Záchia Ayub
BiotecLab, Food Science and Technology Institute, Federal University of Rio Grande do Sul State (UFRGS), Av. Bento Gonçalves, 9500-Campus do Vale, Porto Alegre 91509-900, RS, Brazil
Karina Cesca
Department of Chemical Engineering and Food Engineering, Federal University of Santa Catarina (EQA/UFSC), Campus Reitor João David Ferreira Lima-Centro Tecnológico, Florianópolis 88040-970, SC, Brazil
Paulo Roberto Dall Cortivo
BiotecLab, Food Science and Technology Institute, Federal University of Rio Grande do Sul State (UFRGS), Av. Bento Gonçalves, 9500-Campus do Vale, Porto Alegre 91509-900, RS, Brazil
Cristiano José de Andrade
Department of Chemical Engineering and Food Engineering, Federal University of Santa Catarina (EQA/UFSC), Campus Reitor João David Ferreira Lima-Centro Tecnológico, Florianópolis 88040-970, SC, Brazil
Lilian Raquel Hickert
Materials Recovery and Treatment Laboratory, State University of Rio Grande do Sul State (UERGS), R. Washington Luiz, 675-Centro Histórico, Porto Alegre 90010-460, RS, Brazil
The production of polyhydroxyalkanoates (PHAs) by Bacillus megaterium using industrial residues, crude glycerol from biodiesel synthesis and rice hull hydrolysate (RHH), as low-cost carbon sources was investigated. The experiments were conducted by shaking flasks at 30 °C and 180 rpm up to 72 h. The extraction of PHA was carried out using sodium hypochlorite to make its recovery more environmentally friendly by avoiding organic solvents (chloroform). The yields of PHA varied depending on the extraction method. A total of 33.3% (w·w−1) (mixing chloroform: sodium hypochlorite) and 52.5% (w·w−1) (sodium hypochlorite only) were obtained using glycerol and glucose as a carbon source, respectively. Preliminary experiments using RHH as a carbon source Indicated a yield of PHA of 11% (w·w−1) (chloroform). The PHA produced had thermal properties, such as transition temperature, similar to the commercial polyhydroxybutyrate (PHB).
