Endothelin-1-Induced Persistent Ischemia in a Chicken Embryo Model
Neha Kumari,
Ravi Prakash,
Abu Siddiqui,
Arshi Waseem,
Mohsin Khan,
Syed Raza
Affiliations
Neha Kumari
Laboratory for Stem Cell and Restorative Neurology, Department of Biotechnology, Era’s Lucknow Medical College and Hospital, Era University, Lucknow, Uttar Pradesh, India
Ravi Prakash
Laboratory for Stem Cell and Restorative Neurology, Department of Biotechnology, Era’s Lucknow Medical College and Hospital, Era University, Lucknow, Uttar Pradesh, India
Abu Siddiqui
Laboratory for Stem Cell and Restorative Neurology, Department of Biotechnology, Era’s Lucknow Medical College and Hospital, Era University, Lucknow, Uttar Pradesh, India
Arshi Waseem
Laboratory for Stem Cell and Restorative Neurology, Department of Biotechnology, Era’s Lucknow Medical College and Hospital, Era University, Lucknow, Uttar Pradesh, India
Mohsin Khan
Era’s Lucknow Medical College and Hospital, Era University, Lucknow, Uttar Pradesh, India
Syed Raza
Laboratory for Stem Cell and Restorative Neurology, Department of Biotechnology, Era’s Lucknow Medical College and Hospital, Era University, Lucknow, Uttar Pradesh, IndiaDepartment of Stem Cell Biology and Regenerative Medicine, Era’s Lucknow Medical College and Hospital, Era University, Lucknow, Uttar Pradesh, India
Current ischemic models strive to replicate ischemia-mediated injury. However, they face challenges such as inadequate reproducibility, difficulties in translating rodent findings to humans, and ethical, financial, and practical constraints that limit the accuracy of extensive research. This study introduces a novel approach to inducing persistent ischemia in 3-day-old chicken embryos using endothelin-1. The protocol targets the right vitelline arteries, validated with Doppler blood flow imaging and molecular biology experiments. This innovative approach facilitates the exploration of oxidative stress, inflammatory responses, cellular death, and potential drug screening suitability utilizing a 3-day-old chicken embryo.
