Effects of Film Mulching Cultivation During the Whole Growth Period of Flue-cured Tobacco on Physicochemical Properties and Bacterial Community Structure of Tobacco-planting Soil

Abstract

【Objective】The soil environment of tobacco planting can be impacted by plastic film mulching, which affects the structure of the soil microbial community and the growth and development of flue-cured tobacco. The purpose of this study is to investigate the effect of a full growth period mulching on the physicochemical properties and bacterial community structure of tobacco planting soil.【Method】16S rDNA sequencing was applied to compare the differences in the structure and diversity of bacterial communities in different treatments by various analytical methods. The connection between soil physicochemical properties and bacterial communities was discovered through redundancy analysis (RDA) based on soil physicochemical properties.【Result】The output value of flue-cured tobacco was significantly increased by film mulching during the growth period. The yield was increased by 13.33%, 18.24%, and 21.36% by the double color film mulching (SY), black film mulching (HY), and white film mulching (BY) treatments, respectively, compared to the control (CK); Output value added was 20.26%, 15.87%, and 13.28%. At the same time, film mulching will also increase tobacco-planting soil temperature, total potassium, total phosphorus, total iron and effective copper contents, and decrease soil pH value, alkaline dissolved nitrogen, effective iron, total copper and total zinc contents. The contents of total phosphorus and available copper increased the most significantly, reaching 3.11%-27.33% and 4.76%-19.05%, respectively. The most significant decrease in alkali hydrolyzed nitrogen, available iron, and total zinc occurred at 10.45%-14.50%, 3.36%-13.43%, and 7.69%-10.01%, respectively. Plastic covering during the whole growth period has a certain negative impact on the abundance of bacterial communities. The abundance index Chao 1 of the control was significantly higher than the three mulching treatments and was 14.09%, 24.20%, and 24.81% higher than that of the white film mulching, double color film mulching, and black film mulching treatments, respectively. Redundancy analysis shows that the main soil physicochemical factors affecting the horizontal community structure of the bacterial phylum are soil temperature, alkaline nitrogen, available iron, total iron, available copper, and total copper content. Three shared genera with significantly different abundance from the control were present in the white film mulching and double color film mulching treatments, which belonged to three families in the phylum Proteobacteria: Sphingomonadaceae, Burkholderiaceae, and Xanthomonadaceae.【Conclusion】The full-life stage mulching cultivation will significantly affect the physicochemical properties and bacterial community structure of tobacco-planting soil, and improve the amount of output value of roasted tobacco, suitable for matching the color of the film is black and white bicolor film.

Keywords

• film mulching during the whole growth period
• tobacco-planting soil
• physicochemical properties
• bacterial community structure
• output value
• redundancy analysis