Data on in vivo PGC-1alpha overexpression model via local transfection in aged mouse muscle
Dongwook Yeo,
Chounghun Kang,
Mari Carmen Gomez-Cabrera,
Jose Vina,
Li Li Ji
Affiliations
Dongwook Yeo
Laboratory of Physiological Hygiene and Exercise Science, School of Kinesiology, University of Minnesota Twin Cities, Minneapolis, MN 55455, United States
Chounghun Kang
Department of Physical Education, Inha University, Incheon, South Korea
Mari Carmen Gomez-Cabrera
Department of Physiology, University of Valencia, Investigación Hospital Clínico Universitario/INCLIVA, Valencia, Spain
Jose Vina
Department of Physiology, University of Valencia, Investigación Hospital Clínico Universitario/INCLIVA, Valencia, Spain
Li Li Ji
Laboratory of Physiological Hygiene and Exercise Science, School of Kinesiology, University of Minnesota Twin Cities, Minneapolis, MN 55455, United States; Corresponding author.
The data presented in this article are related to the research paper entitled “Intensified mitophagy in skeletal muscle with aging is downregulated by PGC-1alpha overexpression in vivo” (Yeo et al., 2019). The data explained the surgical procedure of in vivo local transfection by electroporation method in aged mouse tibialis anterior muscle, and plasmid DNA preparation and verification protocol. The data also showed the transfection efficiency levels of GFP or GFP-tagged PGC-1alpha through immunohistochemistry method for frozen muscle cross-sections.
