Antimicrobial, antioxidant, anti-glycation and toxicity studies on silver nanoparticles synthesized using Rosa damascena flower extract

Abstract

The perspective of this research was to investigate the antioxidant, antibacterial, cytotoxic activity and anti-glycation of the SNPs synthesized by aqueous flower extract of pink Rosa damascena plant and compare with the biological effect of plant extract. The antioxidant activity of plant extract, and SNPs, their inhibitory effect on albumin glycosylation and also the cytotoxic effect on the human fibroblast cells were assessed by DPPH, spectrophotometric assay and MTT test, respectively. The antibacterial activity of SNPs against Staphylococcus aureus, Bacillus cereus, Escherichia coli and Klebsiella pneumoniae was examined by the disk diffusion method. The percentage of free radicals inhibited by the extract was increased compared to the SNPs. The flower extract and the SNPs both showed anti-glycation effects under in vitro conditions, but SNPs showed the efficient inhibitory effect of on glycation of BSA in the order; 1000 extract < 500 SNP < 250 SNP < 1000 SNP. The toxicity effects of the extract and SNPs were also found to depend on their concentration and time of incubation, still, SNPs showed 8% higher toxicity than the extract. Although SNPs showed antibacterial activity, their effect on S. aureus was significant. It is concluded that the SNPs exhibited multifunctional properties for many medicinal applications.

Keywords

• rosa damascena plant
• silver nanoparticles
• antibacterial
• antioxidant
• advanced glycation end products (ages)