Multi-Target Antibacterial Mechanism of Moringin From Moringa oleifera Seeds Against Listeria monocytogenes

Yanlong Wen; Yanlong Wen; Yanlong Wen; Wenyun Li; Wenyun Li; Wenyun Li; Rongzhen Su; Min Yang; Min Yang; Min Yang; Nan Zhang; Ximing Li; Ximing Li; Ximing Li; Lingfei Li; Lingfei Li; Lingfei Li; Jun Sheng; Jun Sheng; Yang Tian; Yang Tian; Yang Tian; Yang Tian

doi:10.3389/fmicb.2022.925291

Frontiers in Microbiology (Jun 2022)

Multi-Target Antibacterial Mechanism of Moringin From Moringa oleifera Seeds Against Listeria monocytogenes

Yanlong Wen,
Yanlong Wen,
Yanlong Wen,
Wenyun Li,
Wenyun Li,
Wenyun Li,
Rongzhen Su,
Min Yang,
Min Yang,
Min Yang,
Nan Zhang,
Ximing Li,
Ximing Li,
Ximing Li,
Lingfei Li,
Lingfei Li,
Lingfei Li,
Jun Sheng,
Jun Sheng,
Yang Tian,
Yang Tian,
Yang Tian,
Yang Tian

Affiliations

Yanlong Wen: College of Food Science and Technology, Yunnan Agricultural University, Kunming, China
Yanlong Wen: National Research and Development Professional Center for Moringa Processing Technology, Yunnan Agricultural University, Kunming, China
Yanlong Wen: Engineering Research Center of Development and Utilization of Food and Drug Homologous Resources, Ministry of Education, Yunnan Agricultural University, Kunming, China
Wenyun Li: College of Food Science and Technology, Yunnan Agricultural University, Kunming, China
Wenyun Li: National Research and Development Professional Center for Moringa Processing Technology, Yunnan Agricultural University, Kunming, China
Wenyun Li: Engineering Research Center of Development and Utilization of Food and Drug Homologous Resources, Ministry of Education, Yunnan Agricultural University, Kunming, China
Rongzhen Su: College of Food Science and Technology, Yunnan Agricultural University, Kunming, China
Min Yang: College of Food Science and Technology, Yunnan Agricultural University, Kunming, China
Min Yang: National Research and Development Professional Center for Moringa Processing Technology, Yunnan Agricultural University, Kunming, China
Min Yang: Engineering Research Center of Development and Utilization of Food and Drug Homologous Resources, Ministry of Education, Yunnan Agricultural University, Kunming, China
Nan Zhang: College of Food Science and Technology, Yunnan Agricultural University, Kunming, China
Ximing Li: College of Food Science and Technology, Yunnan Agricultural University, Kunming, China
Ximing Li: National Research and Development Professional Center for Moringa Processing Technology, Yunnan Agricultural University, Kunming, China
Ximing Li: Engineering Research Center of Development and Utilization of Food and Drug Homologous Resources, Ministry of Education, Yunnan Agricultural University, Kunming, China
Lingfei Li: College of Food Science and Technology, Yunnan Agricultural University, Kunming, China
Lingfei Li: Engineering Research Center of Development and Utilization of Food and Drug Homologous Resources, Ministry of Education, Yunnan Agricultural University, Kunming, China
Lingfei Li: Yunnan Engineering Research Center of Drug and Food Homologous Functional Food, Yunnan Agricultural University, Kunming, China
Jun Sheng: National Research and Development Professional Center for Moringa Processing Technology, Yunnan Agricultural University, Kunming, China
Jun Sheng: Engineering Research Center of Development and Utilization of Food and Drug Homologous Resources, Ministry of Education, Yunnan Agricultural University, Kunming, China
Yang Tian: College of Food Science and Technology, Yunnan Agricultural University, Kunming, China
Yang Tian: National Research and Development Professional Center for Moringa Processing Technology, Yunnan Agricultural University, Kunming, China
Yang Tian: Engineering Research Center of Development and Utilization of Food and Drug Homologous Resources, Ministry of Education, Yunnan Agricultural University, Kunming, China
Yang Tian: Yunnan Engineering Research Center of Drug and Food Homologous Functional Food, Yunnan Agricultural University, Kunming, China

DOI: https://doi.org/10.3389/fmicb.2022.925291
Journal volume & issue: Vol. 13

Abstract

Read online

Moringin [4-(α-L-rhamnosyloxy) benzyl isothiocyanate] is an isothiocyanate from Moringa oleifera seeds. It is the bioactivated form of the glucosinolate precursor glucomoringin with various health benefits. However, few studies have examined the antibacterial activity of moringin. This study aimed to investigate the antimicrobial activity and mechanism of moringin against Listeria monocytogenes. The minimum inhibitory concentration (MIC), and growth curves were used to evaluate the bacteriostatic effect of moringin against L. monocytogenes. Transcriptome analysis by RNA sequencing was performed to elucidate the underlying mechanism of moringin against L. monocytogenes. The transcriptome results were validated. The results showed that moringin inhibited the growth of L. monocytogenes with a MIC of 400 μM. RNA sequencing results showed that the differences in the expression of genes related to the cell wall and membrane biosynthesis, phosphotransferase system (PTS), oxidative stress, energy metabolism, and DNA binding were significantly affected. As with the transcriptome results, the results of the mechanism verification found that moringin damaged the integrity of the cell wall and cell membrane, stimulated oxidative stress, interfered with energy metabolism and DNA replication, and finally led to the death of L. monocytogenes. The present study provides evidence that moringin exhibits strong antimicrobial activity against L. monocytogenes and insight into its potential mechanism.

Published in Frontiers in Microbiology

ISSN: 1664-302X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: http://www.frontiersin.org/journals/microbiology

About the journal

Abstract

Keywords