Flow cytometry, a sustainable method for the identification and quantification of microorganisms in enology - Part 1/2 Review of the usual methods applied in wine microbiology and the principle of flow cytometry

Abstract

Flow cytometry (FC) is a powerful technique for the detection, characterization and quantification of microbial populations in enology. Depending on the fluorescent markers and specific probes used, FC can provide information on the physiological state of the cell and allows the quantification of a microorganism of interest, or spoilage microorganisms such as Brettanomyces bruxellensis, within a mixed population.