Avian Haemosporidian Diversity on Sardinia: A First General Assessment for the Insular Mediterranean

Abstract

The Western Palearctic is one of the most investigated regions for avian haemosporidian parasites (Haemoproteus, Plasmodium and Leucocytozoon), yet geographic gaps in our regional knowledge remain. Here, we report the first haemosporidian screening of the breeding birds from Sardinia (the second-largest Mediterranean Island and a biodiversity hotspot), and the first for the insular Mediterranean in general. We examined the occurrence of haemosporidians by amplifying their mtDNA cytb gene in 217 breeding birds, belonging to 32 species. The total prevalence of infected birds was 55.3%, and of the 116 haplotypes recovered, 84 were novel. Despite the high number of novel lineages, phylogenetic analysis did not highlight Sardinia-specific clades; instead, some Sardinian lineages were more closely related to lineages previously recovered from continental Europe. Host-parasite network analysis indicated a specialized host-parasite community. Binomial generalized linear models (GLMs), performed at the community level, suggested an elevational effect on haemosporidian occurrence probability (negative for Haemoproteus; positive for Leucocytozoon) likely due to differences in the abundance of insect vectors at different elevations. Furthermore, a GLM revealed that sedentary birds showed a higher probability of being infected by novel haplotypes and long-distance migrants showed a lower probability of novel haplotype infection. We hypothesize that the high diversity of haemosporidians is linked to the isolation of breeding bird populations on Sardinia. This study adds to the growing knowledge on haemosporidians lineage diversity and distribution in insular environments and presents new insights on potential host-parasite associations.

Keywords

• avian malaria
• genetic diversity
• haemosporidian
• host-parasite association
• MalAvi
• mediterranean