PLoS ONE (Jan 2019)

The effectiveness of colchicine combined with mitomycin C to prolong bleb function in trabeculectomy in rabbits.

  • Taiki Kokubun,
  • Kotaro Yamamoto,
  • Kota Sato,
  • Takahiro Akaishi,
  • Atsushi Shimazaki,
  • Masatsugu Nakamura,
  • Yukihiro Shiga,
  • Satoru Tsuda,
  • Kazuko Omodaka,
  • Toru Nakazawa

DOI
https://doi.org/10.1371/journal.pone.0213811
Journal volume & issue
Vol. 14, no. 3
p. e0213811

Abstract

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PurposeTo investigate the potential of colchicine to improve bleb function after trabeculectomy.MethodsTo find the maximum usable colchicine concentration, an ocular irritation study was performed with the Draize test at concentrations of 0.001%, 0.01% and 0.1%. Additionally, the synergistic effect of topical colchicine instillation and MMC application to surgical site was evaluated in a rabbit model by measuring changes after trabeculectomy in intraocular pressure (IOP) and bleb morphology score at 3, 7, 14, 21, 28, 35, 42, and 49 days.ResultsExperiments with a rabbit model of trabeculectomy showed that 0.04% MMC plus 0.01% colchicine was more effective than saline and 0.04% MMC alone in maintaining IOP reduction at days 7-49 (P ConclusionColchicine may be a promising adjuvant for strengthening the effect of MMC and improving the survival of the filtering bleb in trabeculectomy.