Measuring Laplace pressure and imaging the actin cortex during cytokinesis in cultured cells

Abstract

Summary: Laplace pressure is an important regulator of cell dynamics and behavior during cytokinesis. Here, we provide a protocol to measure Laplace pressure in cultured cells using a micropipette and describe the steps for imaging the actin cortex during cytokinesis. The quantification steps allow tracing dynamic change in Laplace pressure and displaying dynamic response of the actin cortex during cytokinesis in HeLa cells. This protocol can be applied to any cultured cell type during various stages of cell division.For complete details on the use and execution of this protocol, please refer to Wang et al. (2021).

Keywords

• Biophysics
• Cell Biology
• Cell culture
• Single Cell
• Cell-based Assays
• Microscopy