A Flow Cytometric Method to Determine Transfection Efficiency
Wenli Mu,
Stefanie Homann,
Christian Hofmann,
Alexandr Gorin,
Diana Huynh,
Otto Yang,
Theodoros Kelesidis
Affiliations
Wenli Mu
David Geffen School of Medicine, University of California, Los Angeles, USA
Stefanie Homann
David Geffen School of Medicine, University of California, Los Angeles, USA
Christian Hofmann
David Geffen School of Medicine, University of California, Los Angeles, USA
Alexandr Gorin
David Geffen School of Medicine, University of California, Los Angeles, USA
Diana Huynh
David Geffen School of Medicine, University of California, Los Angeles, USA
Otto Yang
David Geffen School of Medicine, University of California, Los Angeles, USA
Theodoros Kelesidis
David Geffen School of Medicine, University of California, Los Angeles, USADepartment of Medicine, Division of Infectious Diseases, David Geffen School of Medicine at UCLA, Los Angeles, California, USA
Mammalian cell transfection is a powerful technique commonly used in molecular biology to express exogenous DNA or RNA in cells and study gene and protein function. Although several transfection strategies have been developed, there is a wide variation with regards to transfection efficiency, cell toxicity and reproducibility. Thus, a sensitive and robust method that can optimize transfection efficiency based not only on expression of the target protein of interest but also on the uptake of the nucleic acids, can be an important tool in molecular biology. Herein, we present a simple, rapid and robust flow cytometric method that can be used as a tool to optimize transfection efficiency while overcoming limitations of prior established methods that quantify transfection efficiency.
