IMPLICATIONS OF INTRATESTICULAR ADMINISTRATION OF ZINC GLUCONATE AND DIMETHYL SULFOXIDE ON TESTICULAR MORPHOMETRY IN DOGS

Abstract

ABSTRACT: A study was performed to investigate the potential contraceptive impact of intra-testicular injection of zinc gluconate with dimethyl sulfoxide (DMSO) with and without local anesthetic in eighteen male mongrel dogs. Animals of Group I and II were treated with 2.62% zinc gluconate plus 0.5% DMSO and 2.62% zinc gluconate plus 0.5% DMSO along with 1% lignocaine hydrochloride solution, respectively. In contrast, Group III animals were maintained as control. The efficacy of combinations was examined by morphometry of testis, scrotal circumference (on day-0, 7, 15, 30), and ultrasonographic examination (on day-0 and 30). The testicular morphometry of both testicles revealed that the testicle size increased significantly on day 7 in the treatment groups and then significantly reduced on days- 15 and 30. The ultrasonographic examination revealed decreased testicular dimensions and increased parenchymal lesions in both the testicles of treatment groups which consisted of heterogenous echotexture, mild evidence of mediastinal line, presence of hypoechogenic areas, and heterogenicity in parenchyma. However, incorporating 1% lignocaine hydrochloride into the zinc gluconate and DMSO did not reveal any appreciable changes during the post-exposure period.

Keywords

• dogs
• chemical sterilization
• zinc gluconate
• dmso
• testicular morphometry