USE OF MODERN STERILIZATION AGENTS FOR IN VITRO GARLIC CULTURE (ALLIUM SATIVUM L.)

Abstract

Aim. The purpose is to study the effectiveness of various disinfectants for the sterilization of the garlic plant material under introduction in vitro culture. Methodology. Sterilization of the plant material was carried out according to a two-stage scheme. Chlorine-containing and quaternary ammonium compounds, as well as water-soluble ‘Glavchlor’ granules and concentrated liquid preparations ‘Deo-bakter’ and ‘Megacid’ were used. The preparations were used in a concentration from 1% to 3% of the active substance and for an exposure time of 20 minutes. Results. Studies have shown that the preparation ‘Glavchlor’ when sterilizing the plant material of garlic, allows one to obtain up to 90% of infection-free explants, and the drug ‘Megacid’ can reduce the level of contamination by 7% - 29%. The use of the drug ‘Deo-bakter’ proved to be ineffective. Research implications. It is proved that ‘Glavchlor’ can be used as an alternative means for sterilization of plant material under introduction in vitro culture.

Keywords

• garlic
• air bulb
• explant
• sterilization