Catastrophic disassembly of actin filaments via Mical-mediated oxidation

Elena E. Grintsevich; Peng Ge; Michael R. Sawaya; Hunkar Gizem Yesilyurt; Jonathan R. Terman; Z. Hong Zhou; Emil Reisler

doi:10.1038/s41467-017-02357-8

Nature Communications (Dec 2017)

Catastrophic disassembly of actin filaments via Mical-mediated oxidation

Elena E. Grintsevich,
Peng Ge,
Michael R. Sawaya,
Hunkar Gizem Yesilyurt,
Jonathan R. Terman,
Z. Hong Zhou,
Emil Reisler

Affiliations

Elena E. Grintsevich: Department of Chemistry and Biochemistry, University of California (UCLA)
Peng Ge: California NanoSystems Institute, UCLA
Michael R. Sawaya: Department of Chemistry and Biochemistry, University of California (UCLA)
Hunkar Gizem Yesilyurt: Departments of Neuroscience and Pharmacology and Neuroscience Graduate Program, The University of Texas Southwestern Medical Center
Jonathan R. Terman: Departments of Neuroscience and Pharmacology and Neuroscience Graduate Program, The University of Texas Southwestern Medical Center
Z. Hong Zhou: California NanoSystems Institute, UCLA
Emil Reisler: Department of Chemistry and Biochemistry, University of California (UCLA)

DOI: https://doi.org/10.1038/s41467-017-02357-8
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 10

Abstract

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MICAL Redox enzymes post-translationally modify F-actin to promote its cellular destabilization. Here, the authors present a 3.9Å cryoEM structure of Mical-oxidized F-actin, showing its nucleotide-state dependent dynamic instability and susceptibility to cofilin-induced severing in the presence of inorganic phosphate.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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