A Generic, Scalable, and Rapid Time-Resolved Förster Resonance Energy Transfer-Based Assay for Antigen Detection—SARS-CoV-2 as a Proof of Concept

Juuso Rusanen; Lauri Kareinen; Leonora Szirovicza; Hasan Uğurlu; Lev Levanov; Anu Jääskeläinen; Maarit Ahava; Satu Kurkela; Kalle Saksela; Klaus Hedman; Olli Vapalahti; Jussi Hepojoki

doi:10.1128/mBio.00902-21

mBio (Jun 2021)

A Generic, Scalable, and Rapid Time-Resolved Förster Resonance Energy Transfer-Based Assay for Antigen Detection—SARS-CoV-2 as a Proof of Concept

Juuso Rusanen,
Lauri Kareinen,
Leonora Szirovicza,
Hasan Uğurlu,
Lev Levanov,
Anu Jääskeläinen,
Maarit Ahava,
Satu Kurkela,
Kalle Saksela,
Klaus Hedman,
Olli Vapalahti,
Jussi Hepojoki

Affiliations

Juuso Rusanen: ORCiD; University of Helsinki, Faculty of Medicine, Medicum, Department of Virology, Helsinki, Finland
Lauri Kareinen: ORCiD; University of Helsinki, Faculty of Medicine, Medicum, Department of Virology, Helsinki, Finland
Leonora Szirovicza: ORCiD; University of Helsinki, Faculty of Medicine, Medicum, Department of Virology, Helsinki, Finland
Hasan Uğurlu: ORCiD; University of Helsinki, Faculty of Medicine, Medicum, Department of Virology, Helsinki, Finland
Lev Levanov: University of Helsinki, Faculty of Medicine, Medicum, Department of Virology, Helsinki, Finland
Anu Jääskeläinen: ORCiD; HUS Diagnostic Center, HUSLAB, Clinical Microbiology, University of Helsinki, Helsinki, Finland
Maarit Ahava: HUS Diagnostic Center, HUSLAB, Clinical Microbiology, University of Helsinki, Helsinki, Finland
Satu Kurkela: HUS Diagnostic Center, HUSLAB, Clinical Microbiology, University of Helsinki, Helsinki, Finland
Kalle Saksela: ORCiD; University of Helsinki, Faculty of Medicine, Medicum, Department of Virology, Helsinki, Finland
Klaus Hedman: ORCiD; University of Helsinki, Faculty of Medicine, Medicum, Department of Virology, Helsinki, Finland
Olli Vapalahti: University of Helsinki, Faculty of Medicine, Medicum, Department of Virology, Helsinki, Finland
Jussi Hepojoki: ORCiD; University of Helsinki, Faculty of Medicine, Medicum, Department of Virology, Helsinki, Finland

DOI: https://doi.org/10.1128/mBio.00902-21
Journal volume & issue: Vol. 12, no. 3

Abstract

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PCR is currently the gold standard for the diagnosis of many acute infections. While PCR and its variants are highly sensitive and specific, the time from sampling to results is measured in hours at best.

Published in mBio

ISSN: 2161-2129 (Print); 2150-7511 (Online)
Publisher: American Society for Microbiology
Country of publisher: United States
LCC subjects: Science: Microbiology
Website: https://journals.asm.org/journal/mbio

About the journal