Lipidomic Characterization of Oocytes at Single-Cell Level Using Nanoflow Chromatography-Trapped Ion Mobility Spectrometry-Mass Spectrometry
Pujia Zhu,
Guowei Bu,
Ruifeng Hu,
Xianqin Ruan,
Rongrong Fu,
Zhourui Zhang,
Qiongqiong Wan,
Xin Liu,
Yiliang Miao,
Suming Chen
Affiliations
Pujia Zhu
The Institute for Advanced Studies, Wuhan University, Wuhan 430072, China
Guowei Bu
Institute of Stem Cell and Regenerative Biology, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China
Ruifeng Hu
Institute of Stem Cell and Regenerative Biology, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China
Xianqin Ruan
The Institute for Advanced Studies, Wuhan University, Wuhan 430072, China
Rongrong Fu
The Institute for Advanced Studies, Wuhan University, Wuhan 430072, China
Zhourui Zhang
The Institute for Advanced Studies, Wuhan University, Wuhan 430072, China
Qiongqiong Wan
The Institute for Advanced Studies, Wuhan University, Wuhan 430072, China
Xin Liu
Institute of Stem Cell and Regenerative Biology, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China
Yiliang Miao
Institute of Stem Cell and Regenerative Biology, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China
Suming Chen
The Institute for Advanced Studies, Wuhan University, Wuhan 430072, China
Mass spectrometry (MS)-based lipidomic has become a powerful tool for studying lipids in biological systems. However, lipidome analysis at the single-cell level remains a challenge. Here, we report a highly sensitive lipidomic workflow based on nanoflow liquid chromatography and trapped ion mobility spectrometry (TIMS)-MS. This approach enables the high-coverage identification of lipidome landscape at the single-oocyte level. By using the proposed method, comprehensive lipid changes in porcine oocytes during their maturation were revealed. The results provide valuable insights into the structural changes of lipid molecules during porcine oocyte maturation, highlighting the significance of sphingolipids and glycerophospholipids. This study offers a new approach to the single-cell lipidomic.
