Jichu yixue yu linchuang (Oct 2020)
HK2 promotes migration and proliferation of human cervical cancer cell lines
Abstract
Objective To investigate the molecular mechanism of HK2 on promoting cell proliferation and migration through p-ERK1/2 in cervical cancer cell line HeLa. Methods Expression of HK2 in cervical cancer cell lines was detected by Western blot and immunocychemistry. Over-expressed HK2 cell line(HeLa-HK2) was established by using G418. Wound-healing and Transwell cells assays were used to detect the migratory and invasive potential in HeLa-HK2 and in control cell lines. Cell growth curves, MTT assay and flow cytometry were used to detect cell proliferation, cell viability and cell cycle. The expression of ERK1/2, p-ERK1/2, cyclin A and Slug was detected by using Western blot and immunocytochemistry. The ERK inhibitor (FR180204) was used to inhibit expression of ERK1/2 and p-ERK1/2, and the potential of cell proliferation, migration and invasion was observed in these cells. Results HeLa-HK2 cells that stably expressing HK2 was successfully constructed. HK2 enhanced cell migration, invasion and growth potential in HeLa cells. The expression of ERK1/2, p-ERK1/2, cyclin A and Slug was higher in HeLa-HK2 cells than those in control cells. The protein level of ERK1/2, p-ERK1/2, cyclin A and Slug was decreased in FR180204 treated HeLa-HK2 cells, and the potential of growth, migratory and invasive were also attenuated by FR180204 in HeLa-HK2 cells. Conclusions HK2 promotes cell growth, migration and invasion in HeLa cells by up-regulating expression of cyclin A and Slug through stimulating p-ERK1/2.
