Quantification of Thrips Damage Using Ilastik and ImageJ Fiji

Isabella  Visschers; Nicole van Dam; Janny Peters

doi:10.21769/BioProtoc.2806

Bio-Protocol (Apr 2018)

Quantification of Thrips Damage Using Ilastik and ImageJ Fiji

Isabella Visschers,
Nicole van Dam,
Janny Peters

Affiliations

Isabella Visschers: Department of Molecular Interaction Ecology, Institute for Water and Wetland Research, Radboud University, 6525 AJ Nijmegen, The Netherlands
Nicole van Dam: Department of Molecular Interaction Ecology, Institute for Water and Wetland Research, Radboud University, 6525 AJ Nijmegen, The NetherlandsGerman Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, Deutscher Platz 5e, 04103 Leipzig, Germany, Friedrich Schiller University Jena, Institute of Ecology, Dornburger-Str. 159, 07743 Jena, Germany
Janny Peters: Department of Molecular Plant Physiology, Institute for Water and Wetland Research, Radboud University, Nijmegen, 6525 AJ Nijmegen

DOI: https://doi.org/10.21769/BioProtoc.2806
Journal volume & issue: Vol. 8, no. 8

Abstract

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Quantification of insect damage is an essential measurement for identifying resistance in plants. In screening for host plant resistance against thrips, the total damaged leaf area is used as a criterion to determine resistance levels. Here we present an objective novel method for analyzing thrips damage on leaf disc using the freely available software programs Ilastik and ImageJ. The protocol was developed in order to screen over 40 Capsicum lines for resistance against Frankliniella occidentalis (Western Flower Thrips) and Thrips tabaci (Onion thrips).

Published in Bio-Protocol

ISSN: 2331-8325 (Online)
Publisher: Bio-protocol LLC
Country of publisher: United States
LCC subjects: Science: Biology (General)
Website: https://bio-protocol.org

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