Bio-Protocol (Apr 2018)

Quantification of Thrips Damage Using Ilastik and ImageJ Fiji

  • Isabella Visschers,
  • Nicole van Dam,
  • Janny Peters

DOI
https://doi.org/10.21769/BioProtoc.2806
Journal volume & issue
Vol. 8, no. 8

Abstract

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Quantification of insect damage is an essential measurement for identifying resistance in plants. In screening for host plant resistance against thrips, the total damaged leaf area is used as a criterion to determine resistance levels. Here we present an objective novel method for analyzing thrips damage on leaf disc using the freely available software programs Ilastik and ImageJ. The protocol was developed in order to screen over 40 Capsicum lines for resistance against Frankliniella occidentalis (Western Flower Thrips) and Thrips tabaci (Onion thrips).