Quantification of Thrips Damage Using Ilastik and ImageJ Fiji
Isabella Visschers,
Nicole van Dam,
Janny Peters
Affiliations
Isabella Visschers
Department of Molecular Interaction Ecology, Institute for Water and Wetland Research, Radboud University, 6525 AJ Nijmegen, The Netherlands
Nicole van Dam
Department of Molecular Interaction Ecology, Institute for Water and Wetland Research, Radboud University, 6525 AJ Nijmegen, The NetherlandsGerman Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, Deutscher Platz 5e, 04103 Leipzig, Germany, Friedrich Schiller University Jena, Institute of Ecology, Dornburger-Str. 159, 07743 Jena, Germany
Janny Peters
Department of Molecular Plant Physiology, Institute for Water and Wetland Research, Radboud University, Nijmegen, 6525 AJ Nijmegen
Quantification of insect damage is an essential measurement for identifying resistance in plants. In screening for host plant resistance against thrips, the total damaged leaf area is used as a criterion to determine resistance levels. Here we present an objective novel method for analyzing thrips damage on leaf disc using the freely available software programs Ilastik and ImageJ. The protocol was developed in order to screen over 40 Capsicum lines for resistance against Frankliniella occidentalis (Western Flower Thrips) and Thrips tabaci (Onion thrips).