Identification of a Major QTL-Controlling Resistance to the Subtropical Race 4 of <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> in <i>Musa acuminata</i> ssp. <i>malaccensis</i>
Andrew Chen,
Jiaman Sun,
Guillaume Martin,
Lesley-Ann Gray,
Eva Hřibová,
Pavla Christelová,
Nabila Yahiaoui,
Steve Rounsley,
Rebecca Lyons,
Jacqueline Batley,
Ning Chen,
Sharon Hamill,
Subash K. Rai,
Lachlan Coin,
Brigitte Uwimana,
Angelique D’Hont,
Jaroslav Doležel,
David Edwards,
Rony Swennen,
Elizabeth A. B. Aitken
Affiliations
Andrew Chen
School of Agriculture and Food Science, The University of Queensland, Brisbane, QLD 4067, Australia
Jiaman Sun
School of Agriculture and Food Science, The University of Queensland, Brisbane, QLD 4067, Australia
Guillaume Martin
CIRAD, UMR AGAP Institut, F-34398 Montpellier, France
Lesley-Ann Gray
Australian Genome Research Facility, Victorian Comprehensive Cancer Centre, Melbourne, VIC 3000, Australia
Eva Hřibová
Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany of the Czech Academy of Sciences, 77200 Olomouc, Czech Republic
Pavla Christelová
Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany of the Czech Academy of Sciences, 77200 Olomouc, Czech Republic
Nabila Yahiaoui
CIRAD, UMR AGAP Institut, F-34398 Montpellier, France
Steve Rounsley
Inari Agriculture, West Lafayette, IN 47906, USA
Rebecca Lyons
School of Agriculture and Food Science, The University of Queensland, Brisbane, QLD 4067, Australia
Jacqueline Batley
School of Biological Sciences, The University of Western Australia, Perth, WA 6907, Australia
Ning Chen
School of Agriculture and Food Science, The University of Queensland, Brisbane, QLD 4067, Australia
Sharon Hamill
Department of Agriculture and Fisheries, Maroochy Research Facility, Nambour, QLD 4560, Australia
Subash K. Rai
Genome Innovation Hub, University of Queensland, Brisbane, QLD 4072, Australia
Lachlan Coin
Department of Microbiology and Immunology, Peter Doherty Institute for Infection and Immunity, University of Melbourne, Melbourne, VIC 3004, Australia
Brigitte Uwimana
International Institute of Tropical Agriculture, Kampala P.O. Box 7878, Uganda
Angelique D’Hont
CIRAD, UMR AGAP Institut, F-34398 Montpellier, France
Jaroslav Doležel
Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany of the Czech Academy of Sciences, 77200 Olomouc, Czech Republic
David Edwards
School of Biological Sciences, The University of Western Australia, Perth, WA 6907, Australia
Rony Swennen
International Institute of Tropical Agriculture, Kampala P.O. Box 7878, Uganda
Elizabeth A. B. Aitken
School of Agriculture and Food Science, The University of Queensland, Brisbane, QLD 4067, Australia
Vascular wilt caused by the ascomycete fungal pathogen Fusarium oxysporum f. sp. cubense (Foc) is a major constraint of banana production around the world. The virulent race, namely Tropical Race 4, can infect all Cavendish-type banana plants and is now widespread across the globe, causing devastating losses to global banana production. In this study, we characterized Foc Subtropical Race 4 (STR4) resistance in a wild banana relative which, through estimated genome size and ancestry analysis, was confirmed to be Musa acuminata ssp. malaccensis. Using a self-derived F2 population segregating for STR4 resistance, quantitative trait loci sequencing (QTL-seq) was performed on bulks consisting of resistant and susceptible individuals. Changes in SNP index between the bulks revealed a major QTL located on the distal end of the long arm of chromosome 3. Multiple resistance genes are present in this region. Identification of chromosome regions conferring resistance to Foc can facilitate marker assisted selection in breeding programs and paves the way towards identifying genes underpinning resistance.
