OncoTargets and Therapy (Dec 2020)
Expression and Prognostic Role of PLOD1 in Malignant Glioma
Abstract
Hao Wang, Weijian Luo, Limeng Dai Department of Neurosurgery, Shenzhen People’s Hospital (Second Clinical Medical College), Ji’nan University, The First Affiliated Hospital, Southern University of Science and Technology, Shenzhen, People’s Republic of ChinaCorrespondence: Limeng DaiSecond Clinical Medical College of Jinan University, No. 1017, Dongmen North Road, Luohu District, Shenzhen 518020, People’s Republic of ChinaEmail [email protected]: Malignant glioma is rarely curable, and factors that influence the prognosis of glioma patients are not fully understood. Lysyl hydroxylases such as PLOD1 promote the cross-linking in extracellular matrix (ECM) molecules, which contribute to ECM structural stability and maturation. However, the expression and prognostic role of PLOD1 in malignant glioma remained to be determined.Methods: The expression of PLOD1 was evaluated by immunohistochemistry in 72 malignant glioma patients from Shenzhen People’s hospital. The mRNA expression profiles and clinical information of malignant glioma patients were obtained from public databases, including TCGA, CGGA, Rembrandt, and Gravendeel. The correlation between gene expression and tumor grade, and IDH1/2 status and 1p19q status were evaluated. The association between gene expression and overall survival of malignant glioma patients was examined using Kaplan–Meier survival analysis. GO and KEGG pathways were analyzed by Metascape. Transwell invasion assays were performed to determine the effect of PLOD1 on migration and invasion of glioma cells in vitro.Results: PLOD1 expression was significantly elevated in malignant glioma tissues compared with non-tumor brain tissues. Besides, elevated levels of PLOD1 were significantly correlated with high tumor grade, wildtype IDH1/2 status, and 1p19q non-codel in all the four public datasets and in-house cohort. Malignant glioma patients with high PLOD1 expression had better overall survival compared to those with low PLOD1 expression. More importantly, patients with IDH1/2 mutations, 1p19q codeletions, and PLOD1 overexpression had the best overall survival. GO enrichment pathway analysis indicated that PLOD1 participates in regulating the extracellular matrix. Transwell invasion assay, which revealed that inhibiting PLOD1 reduced cell invasion in both U87 and U251 cells.Conclusion: PLOD1 serves as a potential prognostic marker and therapeutic target for malignant glioma.Keywords: PLOD1, glioma, prognosis, biomarker, invasion
