Characterization of Nanovesicles Isolated from Olive Vegetation Water
Sandra Buratta,
Raffaella Latella,
Elisabetta Chiaradia,
Anna Maria Salzano,
Brunella Tancini,
Roberto Maria Pellegrino,
Lorena Urbanelli,
Giada Cerrotti,
Eleonora Calzoni,
Husam B. R. Alabed,
Sabrina De Pascale,
Luana Lugini,
Cristina Federici,
Andrea Scaloni,
Carla Emiliani
Affiliations
Sandra Buratta
Department of Chemistry, Biology and Biotechnology, University of Perugia, 06100 Perugia, Italy
Raffaella Latella
Department of Chemistry, Biology and Biotechnology, University of Perugia, 06100 Perugia, Italy
Elisabetta Chiaradia
Department of Veterinary Medicine, University of Perugia, 06100 Perugia, Italy
Anna Maria Salzano
Proteomics, Metabolomics and Mass Spectrometry Laboratory, Institute for the Animal Production System in the Mediterranean Environment (ISPAAM), National Research Council, 80055 Portici, Italy
Brunella Tancini
Department of Chemistry, Biology and Biotechnology, University of Perugia, 06100 Perugia, Italy
Roberto Maria Pellegrino
Department of Chemistry, Biology and Biotechnology, University of Perugia, 06100 Perugia, Italy
Lorena Urbanelli
Department of Chemistry, Biology and Biotechnology, University of Perugia, 06100 Perugia, Italy
Giada Cerrotti
Department of Chemistry, Biology and Biotechnology, University of Perugia, 06100 Perugia, Italy
Eleonora Calzoni
Department of Chemistry, Biology and Biotechnology, University of Perugia, 06100 Perugia, Italy
Husam B. R. Alabed
Department of Chemistry, Biology and Biotechnology, University of Perugia, 06100 Perugia, Italy
Sabrina De Pascale
Proteomics, Metabolomics and Mass Spectrometry Laboratory, Institute for the Animal Production System in the Mediterranean Environment (ISPAAM), National Research Council, 80055 Portici, Italy
Luana Lugini
Department of Oncology and Molecular Medicine, Preclinical Research and Clinical Trials in Hematology and Oncology Unit, Istituto Superiore di Sanità, 00161 Rome, Italy
Cristina Federici
Department of Oncology and Molecular Medicine, Preclinical Research and Clinical Trials in Hematology and Oncology Unit, Istituto Superiore di Sanità, 00161 Rome, Italy
Andrea Scaloni
Proteomics, Metabolomics and Mass Spectrometry Laboratory, Institute for the Animal Production System in the Mediterranean Environment (ISPAAM), National Research Council, 80055 Portici, Italy
Carla Emiliani
Department of Chemistry, Biology and Biotechnology, University of Perugia, 06100 Perugia, Italy
Edible plant and fruit-derived nanovesicles (NVs) are membrane-enclosed particles with round-shape morphology and signaling functions, which resemble mammalian cell-derived extracellular vesicles. These NVs can transmit cross-kingdom signals as they contain bioactive molecules and exert biological effects on mammalian cells. Their properties and stability in the gastrointestinal tract suggest NVs as a promising nutraceutical tool. In this study, we have demonstrated for the first time the presence of NVs in olive vegetation water (OVW), a waste by-product generated during olive oil production. Biophysical characterization by scanning electron microscopy, cryo-transmission electron microscopy, and nanoparticle tracking analysis revealed the presence in OVW of NVs having size and morphology similar to that of vesicles isolated from edible plants. Integrated lipidomic, metabolomic, and proteomic analyses showed that OVW-NVs carry a set of lipids, metabolites and proteins which have recognized antioxidant and anti-inflammatory activities. The nature of biomolecules identified in OVW-NVs suggests that these vesicles could exert beneficial effects on mammalian cells and could be used in the nutraceutical and food industries. The successful isolation of OVW-NVs and the characterization of their features strengthen the idea that agricultural waste might represent a source of NVs having features similar to NVs isolated from edible plants/fruits.
