Guoji Yanke Zazhi (Jul 2020)

Study on the protective effect of quercetin on age-related macular degeneration in mice based on Nrf2/Keap1/ARE pathway

  • Ting-Yan Chen,
  • Yang Zhou

DOI
https://doi.org/10.3980/j.issn.1672-5123.2020.7.04
Journal volume & issue
Vol. 20, no. 7
pp. 1132 – 1138

Abstract

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AIM: To investigate the protective effect and mechanism of quercetin on age-related macular degeneration in mice through Nrf2/Keap1/ARE pathway. METHODS: Kunming mice were used as research objects, which were divided into control group, model group and quercetin group. Fundus examination was showed whether yellow-white like glassy sputum substances appeared in the fundus of each group of mice; OCT was used to examine the retinal thickness of each group of mice; HE staining was used to observe the changes of retinal morphology in each group of mice; FFA was observed the fundus vascular integrity of each group of mice. The activities of SOD, GSH-Px, CAT and the contents of ROS and MDA in serum were detected by ELISA; Western blot was used to detect the expression of Nrf2/Keap1/ARE related proteins in the retina of each group. RESULTS: Quercetin can reduce the yellow and white glassy wart substance in the fundus of mice and increase the thickness of the retina(PPPPPPPCONCLUSION: Quercetin improved the oxidative stress state after retinal photodamage through the Nrf2/Keap1/ARE pathway, protected the retinal function, and protected against age-related macular degeneration.

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