Effects of Hexavalent Chromium on Physiological Function of Heart, Skeletal Muscle and Sciatic Nerve Trunk of Bullfrog

Yanjun ZHANG; Jing ZHANG; Quanyou ZHANG; Mingzhen LI; Pengcui LI; Xiaochun WEI; Weiyi CHEN

doi:10.16355/j.tyut.1007-9432.20230627

Taiyuan Ligong Daxue xuebao (Mar 2024)

Effects of Hexavalent Chromium on Physiological Function of Heart, Skeletal Muscle and Sciatic Nerve Trunk of Bullfrog

Yanjun ZHANG,
Jing ZHANG,
Quanyou ZHANG,
Mingzhen LI,
Pengcui LI,
Xiaochun WEI,
Weiyi CHEN

Affiliations

Yanjun ZHANG: College of Biomedical Engineering, Taiyuan University of Technology, Jinzhong 030600, China
Jing ZHANG: College of Biomedical Engineering, Taiyuan University of Technology, Jinzhong 030600, China
Quanyou ZHANG: College of Biomedical Engineering, Taiyuan University of Technology, Jinzhong 030600, China
Mingzhen LI: College of Mechanical and Vehicle Engineering, Taiyuan University of Technology, Taiyuan 030024, China
Pengcui LI: Shanxi Provincial Key Laboratory for Repair of Bone and Soft Tissue Injury, The Second Hospital of Shanxi Medical University, Taiyuan 030001, China
Xiaochun WEI: Shanxi Provincial Key Laboratory for Repair of Bone and Soft Tissue Injury, The Second Hospital of Shanxi Medical University, Taiyuan 030001, China
Weiyi CHEN: College of Biomedical Engineering, Taiyuan University of Technology, Jinzhong 030600, China

DOI: https://doi.org/10.16355/j.tyut.1007-9432.20230627
Journal volume & issue: Vol. 55, no. 2
pp. 365 – 375

Abstract

Read online

Proposes To investigate the toxicological mechanism of hexavalent chromium, the effects of hexavalent chromium on the heart, gastrocnemius muscle and action potential of sciatic nerve trunk in bullfrog were studied from the perspective of electrophysiology. Methods The bullfrog hearts were treated with different concentrations of potassium dichromate solution by intraperitoneal injection and isolated heart perfusion, and the effects of hexavalent chromium on heart rate and contractility in vivo and in vitro were determined. The gastrocnemius muscle was wrapped with a gauze soaked in potassium dichromate solution, and the contractility of gastrocnemius muscle was measured. The effect of hexavalent chromium on conduction velocity of sciatic nerve trunk was measured by extracellular electrode guidance. Findings The results show that potassium dichromate has an inhibitory effect on heart rate and contractility, and the mechanism may be related to the inhibition of Ttype and L-type calcium channels activity by hexavalent chromium; However, 1 mg/L chromium increases the heart rate of in vivo frog heart after 15 to 30 min, which may be due to the “hormetic effect”; 0.001 to 10 mg/L hexavalent chromium has a concentration-dependent enhancement effect on gastrocnemius contractility, showing a positive inotropic effect, which may be the result of “hormetic effect”; Hexavalent chromium at 100 mg/L inhibits the gastrocnemius contractility, showing a negative inotropic effect, the mechanism is related to the inhibitory effect of hexavalent chromium on ryanodine receptor (RyR); Hexavalent chromium reduces the conduction velocity of sciatic nerve trunk action potential in a concentration and time-dependent manner, and the mechanism was related to the inactivation of voltage-gated Na+ channels by hexavalent chromium. Conclusions Hexavalent chromium has toxicity on heart, skeletal muscle, and nerve, which could provide some electrophysiological experimental basis for the toxicity study of hexavalent chromium.

Published in Taiyuan Ligong Daxue xuebao

ISSN: 1007-9432 (Print)
Publisher: Editorial Office of Journal of Taiyuan University of Technology
Country of publisher: China
LCC subjects: Technology: Chemical technology: Chemical engineering; Technology: Electrical engineering. Electronics. Nuclear engineering: Materials of engineering and construction. Mechanics of materials
Website: https://tyutjournal.tyut.edu.cn/english.html

About the journal

Abstract

Keywords