Development and Validation of HPLC-DAD and UHPLC-DAD Methods for the Simultaneous Determination of Guanylhydrazone Derivatives Employing a Factorial Design
Wanessa Azevedo de Brito,
Monique Gomes Dantas,
Fernando Henrique Andrade Nogueira,
Edeildo Ferreira da Silva-Júnior,
João Xavier de Araújo-Júnior,
Thiago Mendonça de Aquino,
Êurica Adélia Nogueira Ribeiro,
Lilian Grace da Silva Solon,
Cícero Flávio Soares Aragão,
Ana Paula Barreto Gomes
Affiliations
Wanessa Azevedo de Brito
Drug Quality Control Laboratory (LCQMed), Pharmaceutical Sciences Department, Federal University of Rio Grande do Norte–UFRN, Av. General Cordeiro de Faria s/n, Natal RN 59012-570, Brazil
Monique Gomes Dantas
Drug Quality Control Laboratory (LCQMed), Pharmaceutical Sciences Department, Federal University of Rio Grande do Norte–UFRN, Av. General Cordeiro de Faria s/n, Natal RN 59012-570, Brazil
Fernando Henrique Andrade Nogueira
Drug Quality Control Laboratory (LCQMed), Pharmaceutical Sciences Department, Federal University of Rio Grande do Norte–UFRN, Av. General Cordeiro de Faria s/n, Natal RN 59012-570, Brazil
Edeildo Ferreira da Silva-Júnior
Laboratory of Medicinal Chemistry, Nursing and Pharmacy School, Federal University of Alagoas, ESENFAR, Av. Lourival de Mello Motta s/n, Maceió AL 57072-970, Brazil
João Xavier de Araújo-Júnior
Laboratory of Medicinal Chemistry, Nursing and Pharmacy School, Federal University of Alagoas, ESENFAR, Av. Lourival de Mello Motta s/n, Maceió AL 57072-970, Brazil
Thiago Mendonça de Aquino
Laboratory of Medicinal Chemistry, Nursing and Pharmacy School, Federal University of Alagoas, ESENFAR, Av. Lourival de Mello Motta s/n, Maceió AL 57072-970, Brazil
Êurica Adélia Nogueira Ribeiro
Laboratory of Medicinal Chemistry, Nursing and Pharmacy School, Federal University of Alagoas, ESENFAR, Av. Lourival de Mello Motta s/n, Maceió AL 57072-970, Brazil
Lilian Grace da Silva Solon
Pharmaceutical Sciences Department, Federal University of Amapá–UNIFAP, Rod Juscelino Kubitschek, Km-02, Macapá AP 68903-419, Brazil
Cícero Flávio Soares Aragão
Drug Quality Control Laboratory (LCQMed), Pharmaceutical Sciences Department, Federal University of Rio Grande do Norte–UFRN, Av. General Cordeiro de Faria s/n, Natal RN 59012-570, Brazil
Ana Paula Barreto Gomes
Drug Quality Control Laboratory (LCQMed), Pharmaceutical Sciences Department, Federal University of Rio Grande do Norte–UFRN, Av. General Cordeiro de Faria s/n, Natal RN 59012-570, Brazil
Guanylhydrazones are molecules with great pharmacological potential in various therapeutic areas, including antitumoral activity. Factorial design is an excellent tool in the optimization of a chromatographic method, because it is possible quickly change factors such as temperature, mobile phase composition, mobile phase pH, column length, among others to establish the optimal conditions of analysis. The aim of the present work was to develop and validate a HPLC and UHPLC methods for the simultaneous determination of guanylhydrazones with anticancer activity employing experimental design. Precise, exact, linear and robust HPLC and UHPLC methods were developed and validated for the simultaneous quantification of the guanylhydrazones LQM10, LQM14, and LQM17. The UHPLC method was more economic, with a four times less solvent consumption, and 20 times less injection volume, what allowed better column performance. Comparing the empirical approach employed in the HPLC method development to the DoE approach employed in the UHPLC method development, we can conclude that the factorial design made the method development faster, more practical and rational. This resulted in methods that can be employed in the analysis, evaluation and quality control of these new synthetic guanylhydrazones.
