MoS<sub>2</sub>@Au as Label for Sensitive Sandwich-Type Immunoassay of Neuron-Specific Enolase
Yingying Wang,
Huixin Wang,
Yaliang Bai,
Guanhui Zhao,
Nuo Zhang,
Yong Zhang,
Yaoguang Wang,
Hong Chi
Affiliations
Yingying Wang
Shandong Provincial Key Laboratory of Molecular Engineering, School of Chemistry and Chemical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, China
Huixin Wang
Shandong Provincial Key Laboratory of Molecular Engineering, School of Chemistry and Chemical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, China
Yaliang Bai
Shandong Provincial Key Laboratory of Molecular Engineering, School of Chemistry and Chemical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, China
Guanhui Zhao
Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China
Nuo Zhang
Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China
Yong Zhang
Provincial Key Laboratory of Rural Energy Engineering in Yunnan, School of Energy and Environment Science, Yunnan Normal University, Kunming 650500, China
Yaoguang Wang
Shandong Provincial Key Laboratory of Molecular Engineering, School of Chemistry and Chemical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, China
Hong Chi
Shandong Provincial Key Laboratory of Molecular Engineering, School of Chemistry and Chemical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, China
Neuron-specific enolase (NSE) has gained extensive attention as a reliable target for detecting small cell carcinoma of lungs. In this paper, an electrochemical immunoassay method based on molybdenum disulfide (MoS2) is proposed to detect NSE sensitively. By an in-situ growth method, MoS2 and Au nanoclusters (Au NCs) were composited to form a MoS2@Au nanozyme, and then the secondary antibodies were modified. Primary antibodies were immobilized on amino-reduced graphene oxides to capture NSE. The flower-like MoS2 nanozyme provided abundant sites to load Au NCs and catalyze the decomposition of H2O2, which were beneficial to amplify an amperometric response as well as build up sensitivity. Under optimum conditions, the detection range of this strategy was 0.1 pg·mL−1–10 ng·mL−1 and the limit of detection was 0.05 pg·mL−1. This sensing strategy achieved the prospect of sensitively detecting NSE. Moreover, the prepared electrochemical immunosensor provides a theoretical basis and technical support for the detection of other disease markers.
