Perception, regulation, and effects on longevity of pollen fatty acids in the honey bee, Apis mellifera.

Abstract

For successful cross-pollination, most flowering plants rely on insects as pollinators and attract them by offering rewards, predominantly nectar and pollen. Bees-a highly important pollinator group-are especially dependent on pollen as their main source of essential nutrients, including proteins, lipids, and sterols. Fatty acids (FAs) in particular play a pivotal role as fundamental energy source, contributing to membrane structure integrity, cellular homeostasis, and cognitive processes. However, overconsumption of FAs can have detrimental effects on fitness and survival. Thus, bees need to precisely modulate FA intake. To better understand how Apis mellifera, the globally predominant managed pollinator, regulate FA intake, we conducted controlled feeding experiments with newly hatched honey bee workers by providing pollen with different FA concentrations. We additionally investigated the honey bee's capacity to perceive individual FAs by means of chemotactile proboscis extension response (PER) conditioning. We tested both natural concentrations and concentrations exceeding those typically found in pollen. Given the dose-dependent importance of FAs observed in other bee species, we hypothesized that (i) a high FA concentration in pollen would reduce honey bee longevity, and (ii) honey bees are able to perceive individual FAs and differentiate between different FA concentrations via antennal sensation prior to consumption. Our study revealed that elevated FA concentrations in pollen resulted in reduced consumption rates and increased mortality in Apis mellifera. Workers can detect and discriminate between saturated and unsaturated FAs utilizing their antennae. Moreover, they were able to distinguish between individual FAs and also between different concentrations of the same FAs. Our results suggest a high sensitivity of A. mellifera towards both the concentration and composition of individual FAs, which greatly impacts their foraging decisions and fitness. These insights contribute to the growing evidence highlighting the importance of balanced nutrient ratios, in particular of FAs, for bees and other organisms.