Isolation, expansion, differentiation, and histological processing of human nasal epithelial cells

Abstract

Summary: This protocol is intended as a guide for implementing or refining the usage of the air-liquid interface (ALI) model system to generate airway mucociliary tissue in vitro. We present a streamlined protocol for isolating the stem cells from inferior nasal turbinates of donors, allowing for a simple and low-cost supply of primary cells for research. We also provide our detailed protocols for ALI tissue processing and immunofluorescence to aid in the standardization of these techniques between research groups.For complete details on the use and execution of this protocol, please refer to Hussain et al., (2014) Yang et al., (2016) Im et al., (2019).

Keywords

• Cell culture
• Cell isolation
• Microscopy
• Stem cells
• Cell differentiation
• Tissue engineering