HE DEPENDENCE OF IMMUNOGLOBULIN IGA AND IGE TEAR LEVELS FROM GLUTATHIONES-TRANSFERASE P1 GENE POLYMORPHISMS IN STEELWORKERS WITH OPHTHALMOPATHY

Abstract

Abstract. Associations between I105V and A114V gene polymorphisms of glutathione-S-transferase P1 and levels of IgE and secretory IgA in tear fluid were studied in steelworkers with or without ocular diseases, as compared with healthy office employees. Genotyping was performed by means of allele-specific polymerase chain reaction, whereas ELISA technique was used for immune testing. We have found that IgE levels in tear fluid was increased in steelworkers with dystrophic eye diseases, pinguecula/pterygium, and healthy steelworkers, in comparison with control group. This increase correlates with pinguecula/pterygium size and with their industrial employment terms. Secretory IgА levels in tears were decreased in these groups of workers, as compared with controls. Concentrations of both immunoglobulins in tear samples depended on I105V and А114V glutathione-S-transferase P1 gene polymorphisms. I105V polymorphism correlated with lower concentrations of tear IgЕ in steelworkers with ocular dystrophic diseases and healthy metallurgists. А114V polymorphism is connected with decreased quantities of tear IgЕ in steelworkers with ocular dystrophic diseases, as well as with the lower amounts of secretory IgА in healthy workers and administrative staff. Hence, polymorphic loci I105V and А114V of glutathione-S-transferase P1 gene may exert a modulatory effect upon biosynthesis of both Ig types. (Med. Immunol., 2011, vol. 13, N 6, pp 609-616)

Keywords

• ige
• iga
• glutathione-s-transferase p1
• gene polymorphism
• ophthalmic disorders