Effects of cherries Sanitization methods and fermentation times on quality parameters of coffee beans
Andrea Milena Sánchez-Riaño,
Carolina Vega-Oliveros,
Wilmer Licerio Ladino-Garzón,
Dayana Alejandra Orozco-Blanco,
Andrés Felipe Bahamón-Monje,
Nelson Gutiérrez-Guzmán,
Claudia Milena Amorocho-Cruz
Affiliations
Andrea Milena Sánchez-Riaño
Universidad Surcolombiana, Facultad de Ingeniería, Grupo de Investigación Agroindustria USCO, Centro Surcolombiano de Investigación en Café – CESURCAFÉ, Código Postal, 410001, Avenida Pastrana Borrero Carrera 1a, Neiva, Huila, Colombia
Carolina Vega-Oliveros
Universidad Surcolombiana, Facultad de Ingeniería, Grupo de Investigación Agroindustria USCO, Centro Surcolombiano de Investigación en Café – CESURCAFÉ, Código Postal, 410001, Avenida Pastrana Borrero Carrera 1a, Neiva, Huila, Colombia
Wilmer Licerio Ladino-Garzón
Universidad Surcolombiana, Facultad de Ingeniería, Grupo de Investigación Agroindustria USCO, Centro Surcolombiano de Investigación en Café – CESURCAFÉ, Código Postal, 410001, Avenida Pastrana Borrero Carrera 1a, Neiva, Huila, Colombia
Dayana Alejandra Orozco-Blanco
Universidad Surcolombiana, Facultad de Ingeniería, Grupo de Investigación Agroindustria USCO, Centro Surcolombiano de Investigación en Café – CESURCAFÉ, Código Postal, 410001, Avenida Pastrana Borrero Carrera 1a, Neiva, Huila, Colombia
Andrés Felipe Bahamón-Monje
Universidad Surcolombiana, Facultad de Ingeniería, Grupo de Investigación Agroindustria USCO, Centro Surcolombiano de Investigación en Café – CESURCAFÉ, Código Postal, 410001, Avenida Pastrana Borrero Carrera 1a, Neiva, Huila, Colombia
Nelson Gutiérrez-Guzmán
Universidad Surcolombiana, Facultad de Ingeniería, Grupo de Investigación Agroindustria USCO, Centro Surcolombiano de Investigación en Café – CESURCAFÉ, Código Postal, 410001, Avenida Pastrana Borrero Carrera 1a, Neiva, Huila, Colombia
Claudia Milena Amorocho-Cruz
Corresponding author.; Universidad Surcolombiana, Facultad de Ingeniería, Grupo de Investigación Agroindustria USCO, Centro Surcolombiano de Investigación en Café – CESURCAFÉ, Código Postal, 410001, Avenida Pastrana Borrero Carrera 1a, Neiva, Huila, Colombia
For the first time, the dual effect of coffee cherry sanitization methods to control the microbial load in processing and the influence of fermentation time on coffee quality parameters was evaluated. Two assays were carried out by wet processing: I) Sanitization of the coffee cherry (ST1: Unclassified processed cherries; ST2: Classified and sanitized cherries with drinking water; ST3: Classified and sanitized cherries with a chemical agents and II) Fermentation times (FT1: 12 h; FT2: 24 h; FT3: 48 h; FT4: 72 h and FT5: 96 h). pH, temperature, and dissolved oxygen were monitored during fermentation. Counts of Lactic Acid Bacteria - LAB, mesophiles, and yeasts were carried out on the coffee mass before and after fermentation. Caffeine and chlorogenic acid contents were determined by HPLC-DAD and the sensory profile by methodology for specialty coffees (SCA). The main findings showed that: sanitization with Timsen® did not significantly influence the evolution of pH during fermentation (p > 0.05), but it can reduce to a small extent the action of LAB at the end of the process. It was observed that the temperature of the coffee mass tends to balance with the ambient temperature, with significant effects (p < 0.05) of sanitization (ST2 and ST3) on the stability of this variable during fermentation. Timsen® as a disinfectant affected microbial populations and improved the sensory profile in the cup. In prolonged coffee fermentations (FT3, FT4 and FT5), the pH of the coffee mass tended to stabilize after 36 h, regardless of the process time. Likewise, a correlation was evident between a higher microbial load correlated with better sensory profiles in FT4 and FT5. Neither the sanitization process nor the fermentation time significantly affected the caffeine and chlorogenic acid contents of the coffee, both in its green and roasted states. Consequently, the sanitization of cherry coffee with Timsen® and prolonged fermentation times favor the safety and coffee final quality in the cup.
