Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors

Beau R. Webber; Cara-lin Lonetree; Mitchell G. Kluesner; Matthew J. Johnson; Emily J. Pomeroy; Miechaleen D. Diers; Walker S. Lahr; Garrett M. Draper; Nicholas J. Slipek; Branden A. Smeester; Klaus N. Lovendahl; Amber N. McElroy; Wendy R. Gordon; Mark J. Osborn; Branden S. Moriarity

doi:10.1038/s41467-019-13007-6

Nature Communications (Nov 2019)

Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors

Beau R. Webber,
Cara-lin Lonetree,
Mitchell G. Kluesner,
Matthew J. Johnson,
Emily J. Pomeroy,
Miechaleen D. Diers,
Walker S. Lahr,
Garrett M. Draper,
Nicholas J. Slipek,
Branden A. Smeester,
Klaus N. Lovendahl,
Amber N. McElroy,
Wendy R. Gordon,
Mark J. Osborn,
Branden S. Moriarity

Affiliations

Beau R. Webber: Department of Pediatrics, University of Minnesota
Cara-lin Lonetree: Department of Pediatrics, University of Minnesota
Mitchell G. Kluesner: Department of Pediatrics, University of Minnesota
Matthew J. Johnson: Department of Pediatrics, University of Minnesota
Emily J. Pomeroy: Department of Pediatrics, University of Minnesota
Miechaleen D. Diers: Department of Pediatrics, University of Minnesota
Walker S. Lahr: Department of Pediatrics, University of Minnesota
Garrett M. Draper: Department of Pediatrics, University of Minnesota
Nicholas J. Slipek: Department of Pediatrics, University of Minnesota
Branden A. Smeester: Department of Pediatrics, University of Minnesota
Klaus N. Lovendahl: Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota
Amber N. McElroy: Department of Pediatrics, University of Minnesota
Wendy R. Gordon: Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota
Mark J. Osborn: Department of Pediatrics, University of Minnesota
Branden S. Moriarity: Department of Pediatrics, University of Minnesota

DOI: https://doi.org/10.1038/s41467-019-13007-6
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 10

Abstract

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Multiplexed genome engineering with Cas9 can increase efficiency but also the risk of unintended alterations. Here the authors demonstrate the use of multiplexed base editors on primary T cells with reduced translocation frequency.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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