Prussian blue nanofilm-sensitized plasmonic electrochemical microscopy for spatially resolved detection of the localized delivery of hydrogen peroxide

Adaly Garcia; Christina Dhoj; Samuel Groysman; Kinsley Wang; Stellina Ao; Aimee Anguiano; Tony Tran; Dianlu Jiang; Yixian Wang

Sensors and Actuators Reports (Dec 2024)

Prussian blue nanofilm-sensitized plasmonic electrochemical microscopy for spatially resolved detection of the localized delivery of hydrogen peroxide

Adaly Garcia,
Christina Dhoj,
Samuel Groysman,
Kinsley Wang,
Stellina Ao,
Aimee Anguiano,
Tony Tran,
Dianlu Jiang,
Yixian Wang

Affiliations

Adaly Garcia: Department of Chemistry and Biochemistry, California State University, Los Angeles, Los Angeles, CA, USA
Christina Dhoj: Department of Chemistry and Biochemistry, California State University, Los Angeles, Los Angeles, CA, USA
Samuel Groysman: Department of Chemistry and Biochemistry, California State University, Los Angeles, Los Angeles, CA, USA
Kinsley Wang: Department of Chemistry and Biochemistry, California State University, Los Angeles, Los Angeles, CA, USA
Stellina Ao: Department of Chemistry and Biochemistry, California State University, Los Angeles, Los Angeles, CA, USA
Aimee Anguiano: Department of Chemistry and Biochemistry, California State University, Los Angeles, Los Angeles, CA, USA
Tony Tran: Department of Chemistry and Biochemistry, California State University, Los Angeles, Los Angeles, CA, USA
Dianlu Jiang: Department of Chemistry and Biochemistry, California State University, Los Angeles, Los Angeles, CA, USA
Yixian Wang: Corresponding author at: Department of Chemistry and Biochemistry, California State University, Los Angeles, Los Angeles, CA, USA.; Department of Chemistry and Biochemistry, California State University, Los Angeles, Los Angeles, CA, USA

Journal volume & issue: Vol. 8
p. 100218

Abstract

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Hydrogen peroxide (H2O2) sensing has been widely investigated using various electrochemical methods, yet the challenge of finding an imaging technique capable of real-time, spatially resolved detection remains. Addressing this, we introduce a Prussian blue (PB) nanofilm-sensitized plasmonic electrochemical microscopy (PEM) technique that successfully visualizes the localized delivery of H2O2. The PB nanofilm was carefully characterized, and its sensing capability towards H2O2 was demonstrated in amperometric mode. Employing a precise micromanipulator system, we controlled a micropipette to create a localized concentration gradient on the sensor surface and monitored the gradient through the PB nanofilm-sensitized PEM. The accuracy of the obtained concentration values was further validated by numerical simulations based on finite-element methods. Our technique ensures dependable localized detection, and we anticipate that advancements in film uniformity will further improve the resolution. The potential applications of this technique are broad and significant, including the opportunity to investigate single-cell exocytosis with neurotransmitters like dopamine, thus offering a promising avenue for future biomedical research.

Published in Sensors and Actuators Reports

ISSN: 2666-0539 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Mathematics: Instruments and machines
Website: https://www.journals.elsevier.com/sensors-and-actuators-reports/

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