Frontiers in Immunology (Mar 2021)

Long Non-coding RNA GAS5 Regulates T Cell Functions via miR21-Mediated Signaling in People Living With HIV

  • Lam Ngoc Thao Nguyen,
  • Lam Ngoc Thao Nguyen,
  • Lam Nhat Nguyen,
  • Lam Nhat Nguyen,
  • Juan Zhao,
  • Juan Zhao,
  • Madison Schank,
  • Madison Schank,
  • Xindi Dang,
  • Xindi Dang,
  • Dechao Cao,
  • Dechao Cao,
  • Sushant Khanal,
  • Sushant Khanal,
  • Bal Krishna Chand Thakuri,
  • Bal Krishna Chand Thakuri,
  • Zeyuan Lu,
  • Zeyuan Lu,
  • Jinyu Zhang,
  • Jinyu Zhang,
  • Zhengke Li,
  • Zhengke Li,
  • Zheng D. Morrison,
  • Zheng D. Morrison,
  • Xiao Y. Wu,
  • Xiao Y. Wu,
  • Mohamed El Gazzar,
  • Mohamed El Gazzar,
  • Shunbin Ning,
  • Shunbin Ning,
  • Ling Wang,
  • Ling Wang,
  • Jonathan P. Moorman,
  • Jonathan P. Moorman,
  • Jonathan P. Moorman,
  • Zhi Q. Yao,
  • Zhi Q. Yao,
  • Zhi Q. Yao

DOI
https://doi.org/10.3389/fimmu.2021.601298
Journal volume & issue
Vol. 12

Abstract

Read online

T cells are critical for the control of viral infections and T cell responses are regulated by a dynamic network of non-coding RNAs, including microRNAs (miR) and long non-coding RNAs (lncRNA). Here we show that an activation-induced decline of lncRNA growth arrest-specific transcript 5 (GAS5) activates DNA damage response (DDR), and regulates cellular functions and apoptosis in CD4 T cells derived from people living with HIV (PLHIV) via upregulation of miR-21. Notably, GAS5-miR21-mediated DDR and T cell dysfunction are observed in PLHIV on antiretroviral therapy (ART), who often exhibit immune activation due to low-grade inflammation despite robust virologic control. We found that GAS5 negatively regulates miR-21 expression, which in turn controls critical signaling pathways involved in DNA damage and cellular response. The sustained stimulation of T cells decreased GAS5, increased miR-21 and, as a result, caused dysfunction and apoptosis in CD4 T cells. Importantly, this inflammation-driven T cell over-activation and aberrant apoptosis in ART-controlled PLHIV and healthy subjects (HS) could be reversed by antagonizing the GAS5-miR-21 axis. Also, mutation of the miR-21 binding site on exon 4 of GAS5 gene to generate a GAS5 mutant abolished its ability to regulate miR-21 expression as well as T cell activation and apoptosis markers compared to the wild-type GAS5 transcript. Our data suggest that GAS5 regulates TCR-mediated activation and apoptosis in CD4 T cells during HIV infection through miR-21-mediated signaling. However, GAS5 effects on T cell exhaustion during HIV infection may be mediated by a mechanism beyond the GAS5-miR-21-mediated signaling. These results indicate that targeting the GAS5-miR-21 axis may improve activity and longevity of CD4 T cells in ART-treated PLHIV. This approach may also be useful for targeting other infectious or inflammatory diseases associated with T cell over-activation, exhaustion, and premature immune aging.

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