HPLC analysis of anthocyanins and flavonols and expressions of different copies of F3´5´H in grapevine transgenic lines

Abstract

Transgenic lines of grapevine containing silencing constructs of F3´5´H based on ihpRNA mechanism were studied for their flavonoids and for the expression of F3´5´H gene copies. 83-89 % of transgenic lines showed various degrees of silencing, and increase of di-hydroxylated anthocyanins and kaempferol were observed in the leaves of transgenic lines.

Keywords

• Vitis vinifera
• anthocyanin
• flavonol
• F3'5'H
• silencing