Protocol for performing consecutive bone marrow transplants in mice to study the role of marrow niche in supporting hematopoiesis

Abstract

Summary: Hematopoietic stem and progenitor cells depend on bone marrow (BM) stromal cells for survival. Here, we present a protocol for performing three consecutive BM transplants in mice to study the role of BM niche in supporting hematopoiesis. We describe steps for transplanting cells to condition the marrow of the recipient mice and transplanting wild-type cells to examine the effect of the conditioned marrow in supporting hematopoiesis. We then detail procedures for transplanting into wild-type recipients to measure bone marrow chimerism.For complete details on the use and execution of this protocol, please refer to Gopal et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Keywords

• Flow Cytometry
• Cancer
• Model Organisms