Physicochemical Properties of a Bioactive Polysaccharide Film from <i>Cassia grandis</i> with Immobilized Collagenase from <i>Streptomyces parvulus</i> (DPUA/1573)
Kétura Ferreira,
Kethylen Cardoso,
Romero Brandão-Costa,
Joana T. Martins,
Cláudia Botelho,
Anna Neves,
Thiago Nascimento,
Juanize Batista,
Éverton Ferreira,
Fernando Damasceno,
Amanda Sales-Conniff,
Wendell Albuquerque,
Ana Porto,
José Teixeira
Affiliations
Kétura Ferreira
Postgraduate Program in Biological Applied to Health, Department of Biosciences, Federal University of Pernambuco, Recife 50670-901, Brazil
Kethylen Cardoso
Postgraduate Program in Biological Applied to Health, Department of Biosciences, Federal University of Pernambuco, Recife 50670-901, Brazil
Romero Brandão-Costa
Institute of Biological Sciences, University of Pernambuco, Recife 50100-130, Brazil
Joana T. Martins
CEB—Centre of Biological Engineering (LABBELS—Associate Laboratory), University of Minho, 4710-057 Braga, Portugal
Cláudia Botelho
CEB—Centre of Biological Engineering (LABBELS—Associate Laboratory), University of Minho, 4710-057 Braga, Portugal
Anna Neves
Department of Animal Morphology and Physiology, Federal Rural of University of Pernambuco, Recife 52171-900, Brazil
Thiago Nascimento
Campus Professor Cinobelina Elvas, Federal University of Piauí, Bom Jesus 64049-550, Brazil
Juanize Batista
Department of Animal Morphology and Physiology, Federal Rural of University of Pernambuco, Recife 52171-900, Brazil
Éverton Ferreira
Campus Serra da Capivara, Federal University of São Francisco Valley-Univasf, Sao Raimundo Nonato 64770-000, Brazil
Fernando Damasceno
Campus Serra da Capivara, Federal University of São Francisco Valley-Univasf, Sao Raimundo Nonato 64770-000, Brazil
Amanda Sales-Conniff
USF Health Heart Institute, University of South Florida, 560, Channelside Drive, Tampa, FL 33602, USA
Wendell Albuquerque
Institute of Food Chemistry and Food Biotechnology, Justus Liebig University Giessen, Heinrich-Buff-Ring 17, 35392 Giessen, Germany
Ana Porto
Department of Animal Morphology and Physiology, Federal Rural of University of Pernambuco, Recife 52171-900, Brazil
José Teixeira
CEB—Centre of Biological Engineering (LABBELS—Associate Laboratory), University of Minho, 4710-057 Braga, Portugal
(1) Background: Polysaccharide films are promising vehicles for the delivery of bioactive agents such as collagenases, as they provide controlled release at the wound site, facilitating tissue regeneration. This study aimed to investigate the physicochemical properties of Cassia grandis polysaccharide films with immobilized collagenase from Streptomyces parvulus (DPUA/1573). (2) Methods: Galactomannan was extracted from Cassia grandis seeds for film production with 0.8% (w/v) galactomannan and 0.2% (v/v) glycerol with or without collagenases. The films underwent physical-chemical analyses: Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), color and opacity (luminosity-L*, green to red-a*, yellow to blue-b*, opacity-Y%), moisture content, water vapor permeability (WVP), thickness, contact angle, and mechanical properties. (3) Results: The results showed similar FTIR spectra to the literature, indicating carbonyl functional groups. Immobilizing bioactive compounds increased surface roughness observed in SEM. TGA indicated a better viability for films with immobilized S. parvulus enzymes. Both collagenase-containing and control films exhibited a bright-yellowish color with slight opacity (Y%). Mechanical tests revealed decreased rigidity in PCF (−25%) and SCF (−41%) and increased deformability in films with the immobilized bioactive compounds, PCF (234%) and SCF (295%). (4) Conclusions: Polysaccharide-based films are promising biomaterials for controlled composition, biocompatibility, biodegradability, and wound healing, with a potential in pharmacological applications.
