Журнал микробиологии, эпидемиологии и иммунобиологии (Apr 2015)
EVALUATION OF BIOLOGICAL PROPERTIES OF PSEUDOMONAS AERUGINOSA STRAINS ISOLATED FROM PATIENTS WITH STERNUM AND RIB OSTEOMYELITIS
Abstract
Aim. Study of the role of P. aeruginosa in the development of osteomyelitis of sternum and ribs in cardio-surgery patients, and analysis of the main biological properties of the isolated bacterial strains. Materials and methods. 132 bacterial cultures were isolated from 83 hospital patients as a result of bacteriological examination during 2007 - 2013. Wound discharge was the study material. Sampling, seeding and identification of the isolated cultures was carried out by using the respective test-systems; antibiotic sensitivity was studied by disc-diffusion method. Results. The proportion of P. aeruginosa was 10.6% (n=14) that is comparable with data on wound infections of general surgery hospitals. A direct and strong correlation (R=0.846, p=0.000132) between hemolytic and phospholipase activity was established during evaluation of virulence properties of the isolated strains. The degree of film-forming ability varied significantly from 0.122 to 1.412 OD; 64.3% of the studied cultures were highly film-forming variants. Statistically significant association between biofilm formation and other studied properties was not found. 4 strains produced VIM2-type metallo-betalactamase and had identical RAPD profiles. Conclusion. Considering that earlier the similar cultures were not detected and all of them were isolated at a short interval of time, we have made a conclusion, that their short-term circulation is probably associated with introduction, which was the reason for patient infection. P. aeruginosa could be the etio-pathogen of both early and later complications of cardio-surgical interventions.
