Optimization of Resolving Power, Fragmentation, and Mass Calibration in an Orbitrap Spectrometer for Analysis of 24 Pesticide Metabolites in Urine

Pablo Dualde; Clara Coscollà; Agustin Pastor; Vicent Yusà

doi:10.1155/2019/1917369

International Journal of Analytical Chemistry (Jan 2019)

Optimization of Resolving Power, Fragmentation, and Mass Calibration in an Orbitrap Spectrometer for Analysis of 24 Pesticide Metabolites in Urine

Pablo Dualde,
Clara Coscollà,
Agustin Pastor,
Vicent Yusà

Affiliations

Pablo Dualde: Foundation for the Promotion of Health and Biomedical Research in the Valencian Region, FISABIO-Public Health, 21 Avenida Catalunya, 46020 Valencia, Spain
Clara Coscollà: Foundation for the Promotion of Health and Biomedical Research in the Valencian Region, FISABIO-Public Health, 21 Avenida Catalunya, 46020 Valencia, Spain
Agustin Pastor: Analytical Chemistry Department, University of Valencia, Edifici Jeroni Muñoz, Dr. Moliner 50, 46100 Burjassot, Spain
Vicent Yusà: Foundation for the Promotion of Health and Biomedical Research in the Valencian Region, FISABIO-Public Health, 21 Avenida Catalunya, 46020 Valencia, Spain

DOI: https://doi.org/10.1155/2019/1917369
Journal volume & issue: Vol. 2019

Abstract

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Mass spectrometer parameters such as Resolving Power, type of fragmentation, and mass calibration mode were optimized in the analysis of 24 pesticide metabolites in human urine using Ultra-High Pressure Liquid Chromatography coupled to Orbitrap High-Resolution Mass Spectrometer (UHPLC-HRMS). The best results were achieved with a Resolving Power of 25,000 FWHM and by applying Collision Induced Dissociation fragmentation mode (40 eV).

Published in International Journal of Analytical Chemistry

ISSN: 1687-8760 (Print); 1687-8779 (Online)
Publisher: Wiley
Country of publisher: United Kingdom
LCC subjects: Science: Chemistry: Analytical chemistry
Website: https://onlinelibrary.wiley.com/journal/7072

About the journal