OncoTargets and Therapy (Dec 2019)
EPS8 is a Potential Oncogene in Glioblastoma
Abstract
Gang Yang,1,2 Yong-bin Lu,3 Quan-lin Guan2,4 1Department of Neurosurgery, The First Hospital of Lanzhou University, Lanzhou, Gansu 730000, People’s Republic of China; 2The First Clinical Medical College of Lanzhou University, Lanzhou, Gansu 730000, People’s Republic of China; 3Department of Technology, The First Hospital of Lanzhou University, Lanzhou, Gansu 730000, People’s Republic of China; 4Department of Oncology Surgery, The First Hospital of Lanzhou University, Lanzhou, Gansu 730000, People’s Republic of ChinaCorrespondence: Quan-lin GuanDepartment of Oncology Surgery, The First Hospital of Lanzhou University, Donggang West Road, Chengguan District, Lanzhou, Gansu Province 730000, People’s Republic of ChinaTel|Fax +86 931 861 9797Email [email protected]: In this study, we investigated the expression and function of Epidermal growth factor receptor kinase substrate 8 (EPS8) in glioblastoma (GBM), and further explored the underlying mechanisms that regulate it.Patients and methods: The expression and potential mechanisms of EPS8 in GBM were evaluated through multiple online public databases. The expression level EPS8 in GBM tissues and cell lines were detected by immunohistochemical staining and Western blot. Then, the prognosis of EPS8 and GBM patients were analyzed. Loss-of-function experiments were conducted to determine the role of EPS8 for the biological behavior of GBM cells. In addition, the tumorigenic ability of nude mice was tested in vivo.Results: EPS8 is highly expressed in GBM tissues and indicates poor patient prognosis. In cell experiments, EPS8 can promote the proliferation, migration and invasion of GBM cells. In vivo, EPS8 promotes tumor formation in nude mice. EPS8 can activate the PI3K/Akt signaling pathway to function.Conclusion: EP8S plays a role in the development of GBM and may be a potential therapeutic target for GBM.Keywords: GBM, EPS8, PI3K/Akt pathway
