Lineage Tracing and Molecular Real-Time Imaging of Cancer Stem Cells
Xiaohua Jia,
Guodong Shen,
Jia Jia,
Yan Zhang,
Dan Zhang,
Wanjun Li,
Jianjun Zhang,
Xinglu Huang,
Jie Tian
Affiliations
Xiaohua Jia
Key Laboratory of Molecular Imaging of Chinese Academy of Sciences, Institute of Automation, Chinese Academy of Sciences, Beijing 100190, China
Guodong Shen
Department of General Surgery & Guangdong Provincial Key Laboratory of Precision Medicine for Gastrointestinal Tumor, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China
Jia Jia
Fenyang College, Shanxi Medical University, Fenyang 032200, China
Yan Zhang
Department of Ultrasound, General Hospital of People’s Liberation Army, Beijing 100853, China
Dan Zhang
Center of Biomedical Analysis, Tsinghua University, Beijing 100084, China
Wanjun Li
Department of Pathology, Affiliated 3201 Hospital of Xi’an Jiaotong University, Hanzhong 723000, China
Jianjun Zhang
Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences, Beijing 100101, China
Xinglu Huang
Key Laboratory of Bioactive Materials for the Ministry of Education, College of Life Sciences, State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300071, China
Jie Tian
Key Laboratory of Molecular Imaging of Chinese Academy of Sciences, Institute of Automation, Chinese Academy of Sciences, Beijing 100190, China
The cancer stem cells (CSC) are the roots of cancer. The CSC hypothesis may provide a model to explain the tumor cell heterogeneity. Understand the biological mechanism of CSC will help the early detection and cure of cancer. The discovery of the dynamic changes in CSC will be possible by the using of bio-engineering techniques-lineage tracing. However, it is difficult to obtain real-time, continuous, and dynamic live-imaging information using the traditional approaches that take snapshots of time points from different animals. The goal of molecular imaging is to monitor the in situ, continuous molecular changes of cells in vivo. Therefore, the most advanced bioengineering lineage tracing approach, while using a variety of molecular detection methods, will maximize the presentation of CSC. In this review, we first introduce the method of lineage tracing, and then introduce the various components of molecular images to dynamic detect the CSC. Finally, we analyze the current situation and look forward the future of CSC detection.
