Oral Immunity and Inflammation Unit, NIDCR, NIH, Bethesda, MD, USA
Bo-Young Hong
Division of Periodontology, Department of Oral Health and Diagnostic Sciences, UConn Health, Farmington, CT, USA
Anilei Hoare
Division of Periodontology, Department of Oral Health and Diagnostic Sciences, UConn Health, Farmington, CT, USA
Joanne Konkel
Faculty of Biology, Medicine and Health, University of Manchester, Manchester, UKManchester Collaborative Centre for Inflammation Research (MCCIR), University of Manchester, Manchester, UK
Patricia Diaz
Division of Periodontology, Department of Oral Health and Diagnostic Sciences, UConn Health, Farmington, CT, USA
Niki Moutsopoulos
Oral Immunity and Inflammation Unit, NIDCR, NIH, Bethesda, MD, USA
The oral microbiome has been implicated as a trigger for immune responsiveness in the oral cavity, particularly in the setting of the inflammatory disease periodontitis. The protocol presented here is aimed at characterizing the oral microbiome in murine models at steady state and during perturbations of immunity or physiology. Herein, we describe murine oral microbiome sampling procedures, processing of low biomass samples and subsequent microbiome characterization based on 16S rRNA gene sequencing.
