Effect of C-CSN and B-CSN genotypes on milk production traits in Czech Flekvieh and Holstein breed

Abstract

The effect of the χ-casein (C-CSN) and β –casein (B-CSN) loci on the milk production traits (milk yield, fat, protein and lactose content) was estimated for 807 and 488 Czech Fleckvieh and 402 and 244 Holstein cows, respectively. Genotypes of C-CNS and B-CNS were determined by the use of PCR–RFLP method. The genotypes were detected by use of electrophoresis on agarose gel. The associations of studied polymorphisms with milk production traits were estimated using the mixed linear model procedure REML in SAS for Windows 9.1.3. Results indicated that protein content is significantly affected (P ≤ 0.01) by C-CSN genotype (genotype BB > AB > AA). Fat and lactose content were not affected by C-CSN locus. The B-CNS locus had no significant effect on any milk production traits.

Keywords

• χ–casein
• β-casein
• Czech Fleckvieh
• Holstein cows