Single-cell multiomics reveals the complexity of TGFβ signalling to chromatin in iPSC-derived kidney organoids

Jessica L. Davis; Ciaran Kennedy; Shane Clerkin; Niall J. Treacy; Thomas Dodd; Catherine Moss; Alison Murphy; Derek P. Brazil; Gerard Cagney; Dermot F. Brougham; Rabi Murad; Darren Finlay; Kristiina Vuori; John Crean

doi:10.1038/s42003-022-04264-1

Communications Biology (Nov 2022)

Single-cell multiomics reveals the complexity of TGFβ signalling to chromatin in iPSC-derived kidney organoids

Jessica L. Davis,
Ciaran Kennedy,
Shane Clerkin,
Niall J. Treacy,
Thomas Dodd,
Catherine Moss,
Alison Murphy,
Derek P. Brazil,
Gerard Cagney,
Dermot F. Brougham,
Rabi Murad,
Darren Finlay,
Kristiina Vuori,
John Crean

Affiliations

Jessica L. Davis: UCD School of Biomolecular and Biomedical Science, UCD Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield
Ciaran Kennedy: UCD School of Biomolecular and Biomedical Science, UCD Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield
Shane Clerkin: UCD School of Biomolecular and Biomedical Science, UCD Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield
Niall J. Treacy: UCD School of Biomolecular and Biomedical Science, UCD Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield
Thomas Dodd: UCD School of Biomolecular and Biomedical Science, UCD Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield
Catherine Moss: UCD Genomics Core Facility, UCD Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield
Alison Murphy: UCD Genomics Core Facility, UCD Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield
Derek P. Brazil: Wellcome-Wolfson Institute for Experimental Medicine, Queen’s University Belfast
Gerard Cagney: UCD School of Biomolecular and Biomedical Science, UCD Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield
Dermot F. Brougham: UCD School of Chemistry, University College Dublin, Belfield
Rabi Murad: Sanford Burnham Prebys Institute for Medical Discovery
Darren Finlay: Sanford Burnham Prebys Institute for Medical Discovery
Kristiina Vuori: Sanford Burnham Prebys Institute for Medical Discovery
John Crean: UCD School of Biomolecular and Biomedical Science, UCD Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Belfield

DOI: https://doi.org/10.1038/s42003-022-04264-1
Journal volume & issue: Vol. 5, no. 1
pp. 1 – 16

Abstract

Read online

Multimodal single-cell analysis on iPSC-derived kidney organoids shows that inhibiting EZH2 attenuates TGFβ1-induced fibrotic gene expression and changes in chromatin accessibility, highlighting a potential therapeutic strategy for renal fibrosis.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

About the journal