Minimum lethal dose of the herbicide BASTA® on in vitro plants of the papaya hybrid IBP 42-99

Abstract

All the transformation systems developed up to the moment need to select those plants that contain the transgene introduced and eliminate the rest. One way is to use as scoreboard a resistance gene to an herbicide. The main objective was to study the lethal minimal dose of the herbicide BASTAR an in vitro plants hybrid IBP 42-99. In vitro plants of the papaya hybrid IBP 42-99 were used as plant material, which were in 6th subculture. MS culture medium supplement with 6-BAP 0.45 mg.l-1 and ANA 0.14 mg.l-1, sucrose 3 % was used and hardened with gelrite 2.5 g.l-1, the pH was always adjusted to 5.8 before autoclaved. (BASTA®) was used as selective agent in the following concentrations (0, 5, 10, 15 mg.l-1). The best result were achieved when 10 mg.l-1 were used since it was the treatment with minor concentration of the herbicide that produced the 100 % of mortality in the in vitro plants. Key words: biotechnology, Carica papaya, in vitro plants, selection, culture medium