Nature Communications (Mar 2022)

A deep-learning approach for online cell identification and trace extraction in functional two-photon calcium imaging

  • Luca Sità,
  • Marco Brondi,
  • Pedro Lagomarsino de Leon Roig,
  • Sebastiano Curreli,
  • Mariangela Panniello,
  • Dania Vecchia,
  • Tommaso Fellin

DOI
https://doi.org/10.1038/s41467-022-29180-0
Journal volume & issue
Vol. 13, no. 1
pp. 1 – 22

Abstract

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Processing of two-photon calcium imaging data is generally time-consuming, especially for large fields of view. Here, the authors present CITE-On, a tool based on a convolutional neural network, enabling online automatic cell identification, segmentation, identity tracking, and trace extraction.